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Biological contact oxidation reactor gravel dominant flora FISH evaluation

Author: YangYinZhu
Tutor: LinShanShan
School: Northeast Normal University
Course: Environmental Engineering
Keywords: Fluorescence in situ hybridization Excess sludge Dominant flora Bio gravel contact oxidation reactor Confidence interval
CLC: X703
Type: Master's thesis
Year: 2009
Downloads: 94
Quote: 1
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Abstract


Sludge has been a lot of emissions of organic biological wastewater treatment process of the problem, along with the increase in the amount of processing and handling the increasing demand indicators, makes this problem even more urgent. In order to achieve excess sludge, people from the physical, chemical and biological point of view were carried out exploration and research. Through continuous research people, found that the most simple and economical biological method, and less pollution. According to microbial growth and the relationship between law, the principle of excess sludge mainly include energy uncoupling, maintain energy and endogenous respiration, small animal prey, such as slow-growing bacteria predominate. In order to achieve excess sludge reduction, study microbial diversity, dominance and growth kinetics of bacteria is particularly important. In this study, fluorescence in situ hybridization (FISH) technology on behalf of the sewage treatment process has the role of β-Proteobacteria, ε-Proteobacteria, Bacteroides, raw disk fiber hair bacteria (Leptothrix discophora), Xanthomonas campestris (Xanthomonas branch ) five types of bacteria to conventional activated sludge biological contact oxidation gravel parallel controlled way to compare the distribution characteristics of dominant flora and quantity characteristics, and further confirmation from the mechanism of functional residual sludge reduction whether bacteria work, explore the dominant flora distribution characteristics to identify bacteria may play a role in sludge reduction. In this study, the advantages of the two systems than on the distribution of flora and further explore the biological contact oxidation reaction process gravel sludge removal mechanism, as well as dominant flora and excess sludge reduction and regulation of the correlation. Explore the use of means to reduce excess sludge microbial method to reduce the total cost of sewage treatment, to further improve on the microbial degradation of pollutants and improve the efficiency of biological treatment of organic wastewater, has important theoretical and practical significance. Through the study found biological contact oxidation process than ordinary gravel activated sludge process has a relatively complex living environment. The central limit theorem using statistical and maximum likelihood estimation theorem, analysis confidence interval for the number of bacteria, obtained ε-Proteobacteria and Bacteroides number of developmental biological gravel contact oxidation reactor is conventional activated sludge reactor 3.5 and 2 times, which may be caused by the new reflection is the root cause of zero discharge of sludge. Recommended that in future research work should increase the dominant strain and kinetic analysis matrix interactions, from the microbiological point of view, in-depth study of excess sludge reduction mechanism.

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CLC: > Environmental science, safety science > Processing and comprehensive utilization of waste > General issues > Wastewater treatment and utilization
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