Dissertation > Excellent graduate degree dissertation topics show

A Study on ALK Fusion Gene and Nasopharyngeal Carcinoma

Author: LiuZhiMin
Tutor: ZengYiXin
School: Beijing Union Medical College
Course: Clinical
Keywords: nasopharyngeal carcinoma anaplastic lymphoma kinase fusiongene fluorescence in situ hybridization immunohistochemistry
CLC: R739.63
Type: PhD thesis
Year: 2012
Downloads: 52
Quote: 0
Read: Download Dissertation

Abstract


[Objective] To study the rearrangement of the anaplastic lymphoma kinase(ALK) gene and the expression of ALK in nasopharyngeal carcinoma(NPC),and to investigate the correlation between ALK gene rearrangement and NPC.[Methods] Ninety-nine cases of NPC biopsies of northern Chinese patients, from2006to2009, were collected from the Department of Pathology, Cancer Hospital of Chinese Academy of Medical Science. All cases were formalin-fixed and paraffin-embedded. A recently developed ALK(D3F5) monoclonal antibody was used to assess ALK fusion protein expression using immunohistochemistry (IHC). Fluorescence in situ hybridization(FISH) was also performed on the neoplastic cells to detect ALK gene abnormality.[Results] ALK fusion protein expression was found in1of99cases(1.0%).In this case, ALK was expressed in cytoplasm. A sample is considered positive if≥15%tumor cells shows splitting signals in FISH analysis. According to this criteria, the detection rate of ALK gene rearrangement is1.0%(1/99) in NPC. The result of FISH is concordant to IHC.[Conclusion] ALK gene rearrangement and protein expression shows a extremely low incidence rate in NPC. Whether ALK fusion gene is related to NPC remains uncertain.

Related Dissertations

  1. Roles of MMP-7 and Lysozyme in the Pathogenesis of Ulcerative Colitis Induced by DSS in Balb/c Mice,S858.91
  2. The Study of Kisspeptin/GPR54 System on the Reproductive Regulation in the Hen’s Hypothalamus-Pituitary-Ovary Axis,S831
  3. Study on the Digestive Tube During the Embryonic Stage of Gushi Chicken,S831
  4. A Comparative Study of prawns, crabs, Spiroplasma disease immunohistochemistry and pathology,S945
  5. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  6. Expressions of PRL-3 and p27 in Gastric Carcinoma and Their Clinical Significance,R735.2
  7. Expression of hnRNP A2/B1 and p21WAF1 Proteins in Lung Cancer and Their Clinical Significance,R734.2
  8. The Effects of RIP on the Immune Function of Renewal Rats Without Colostrums,S853.7
  9. Study on the Expression and Correlation of Metallothionein and Matrix Metalloproteinase-2 in Non-small Cell Lung Cancer,R734.2
  10. Expression and Their Significance of Apoptosis Regulatory Factors Survivin、XIAP and c-Jun、NF-κB in Epithelial Neoplasm of Ovary,R737.31
  11. Expression and Clinical Significance of Survivin and YAP in Cervical Carcinoma,R737.33
  12. Expression and Clinical Significance of PTEN and Caspase-7 in Gastric Cancer and Precancerous Lesion,R735.2
  13. Research for the Expressive Correlation of EBERs and BMI-1 in Nasopharyngeal Carcinoma,R739.63
  14. Cloning and Expression Analysis of NCCRP-1 AND IL-10 Genes in Grass Carp (Ctenopharyngodon Idellus),S917.4
  15. Expression and Antibody Preparation of sglt1 from Cyprinus CarpioL.,S917.4
  16. The Effect of Hepatic Denervation on Liver Regeneration after Partially Hepatectomy in Rats,R363
  17. Expression and Correlation of Caspase-3 and Cx43 in Myocardial Infarction with Different Narrow Degree Coronary Artery,R542.22
  18. The Expression and Relationships of Bmi-1 and c-myc in Diffuse Large B-cell Lymphoma Tissues,R733.1
  19. Expression of Caspase-4 and Caspase-3 Gene Related Apoptosis in Pathological Changes of Sternocleidomastoid of Congenital Muscular Torticollis and Four Years Clinical Followed-up for Opereated Patients,R726.8
  20. The Feasibility of the Immunohistochemistry Classification to Predicting Prognosis in Breast Cancer,R737.9
  21. Clonal Origin of Multifocal Clear Cell Renal Cellcarcinoma Studied by Fluorescence in Situ Hybridization,R737.11

CLC: > Medicine, health > Oncology > Department of Otolaryngology tumor > Pharyngeal tumors
© 2012 www.DissertationTopic.Net  Mobile