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Objective: In this study, by using a laser scanning confocal microscope for different obturation techniques in the formation of micro-leakage after filling observe and measure the effects of different techniques to form filling microleakage situation in order to select the appropriate period for the clinical experiment filling technology reference data. Methods: 40 due to removal of healthy adolescents orthodontic permanent premolar in the occlusal surface preparation 4.0mm × 3.0mm × 2.0mm hole in a box-like shape. Randomly divided into A, B groups, A group was randomly divided into A1, A2 two sub-group, B group were randomly divided into B1, B2 two subgroups. A group using silver amalgam as a filling material, A1 group bonded prostheses using silver amalgam filling; A2 group with direct amalgam filling. Group B using a composite resin as a filling material, B1 group with self-etching bonding technology, B2 group with total-etch bonding technique. After filling material to be cured, will be fully equipped with the specimen placed in saline at 37 ℃ water bath for 1 day, during thermal cycling experiments conducted, that alternately placed 5 ℃ and 55 ℃ water each 30 seconds 500 cycles . All experimental teeth will be dry, hole-shaped around 1.0 mm in outer dental office uniform coating layer 2 nail polish, self-curing plastic closures apical foramen. All the specimens immersed at 37 ℃ for 0.1% rhodamine B isothiocyanate alcohol solution for 24 hours, rinse thoroughly with distilled water, the use of low-speed diamond film specimen along the direction of the filling area buccal and lingual continuous slicing 2, thickness of 1.5mm, with different size from coarse to fine oil stone slicing specimens were polished until translucent specimens. The specimen placed on a slide, maintain 100% relative humidity, at an excitation wavelength of 546nm and emission wavelength of 600nm, using the confocal laser scanning microscope microleakage case, the length of the measurement for statistical analysis. Results: The dental filling materials with varying degrees of interfacial dye penetration. Staining depth measurement results showed that: the use of amalgam bonding repair surgery group (A1) microleakage depth of 20.54 ± 5.38μm, traditional amalgam filling group (A2) microleakage depth of 35.71 ± 9.97μm its significant difference was statistically significant (P lt; 0.05); when using self-etching bonding technology group (B1) microleakage depth of 60.03 ± 7.83μm and total-etch bonding technique group (B2) of microleakage depth of 46.78 ± 10.28μm, the results also showed significant differences were statistically significant (P lt; 0.05). Conclusions: 1 using silver amalgam bonding prosthetics and traditional amalgam filling, the traditional way of direct filling microleakage produced larger value. (2) using self-etch and total-etch bonding technique bonded composite resin bonding techniques, self-etching adhesive composite resin technology microleakage larger value.
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