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Objective: In this study, using human dermal fibroblasts as an experimental model to study sphingosine phosphorylcholine (sphingosylphosphorylchoine, SPC) on human dermal fibroblast matrix metalloproteinase -1 (MMP-1), matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase -3 (MMP-3) expression , and to explore SPC promote skin wound healing mechanism. Methods: Using tissue digestion dermal fibroblasts cultured , take 4-10 generations to experiment by reverse transcription polymerase chain reaction (RT-PCR) and immunoblotting (Western blotting) semi-quantitative detection method induced by SPC human dermal fibroblasts MMP-1, MMP-2, MMP-3 mRNA levels and protein expression levels . Results: The use of tissue digestion dermal fibroblasts cultured with the traditional method of comparison digestion and tissue , the success rate is higher. SPC to time gradient ( 2,6,12,24 and 48 hours ) induced human dermal fibroblasts , RT-PCR showed that , mRNA expression levels produced different degrees of change , MMP-1, MMP-3 in the SPC role in two hours or so gradually increased, peaked around six hours , for some time after the fall. MMP-2 mRNA expression levels did not change significantly . Western blotting analysis showed that , SPC for MMP-1, MMP-3 protein levels in the same time-dependent , MMP-2 protein expression did not change significantly . Conclusion : SPC promoting human dermal fibroblasts MMP-1, MMP-3 expression , and thus participate in the regulation of the extracellular matrix , which may be SPC promote skin wound healing mechanisms.
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