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Periodontal disease is a common oral diseases and frequently-occurring disease, its etiology was mainly due to direct damage to the periodontal tissues of some bacteria, but these bacteria by producing toxins and their antigen-induced periodontal tissue localized immune cells produce cytokines, increased tooth peripheral inflammation. Actinomyces within the bacteria associated with periodontal disease, which can induce cells produce large amounts of tumor necrosis factor TNF-α, leading to cell necrosis, collapse, direct damage to the periodontal tissues. Cinnamic aldehyde cinnamon extract, cinnamaldehyde recently found that some bacteria and can inhibit IL-1β, TNF-α production. But whether it can inhibit periodontal disease associated with Actinomyces within lipoteichoic acid (LTA) induced by periodontal ligament fibroblasts produce TNF-a is unclear. Objective: This study was to observe whether the inhibition of cinnamic aldehyde Actinomyces growth; determination of cinnamaldehyde Actinomyces internal LTA stimulation of periodontal ligament fibroblasts to produce TNF-α effect, see its ability to inhibit the LTA induced periodontal ligament fibroblasts to produce TNF-α. Cinnamaldehyde to study the effect in the treatment of periodontitis aspects of research, to find a drug treatment of periodontitis provide theoretical and experimental evidence. Method: The in vitro antibacterial test method and cell culture: an inner strain ATCC12104 Actinomyces dubbed 5 × 103 cells / ml. The cinnamic aldehyde solution and metronidazole were diluted 1024,512,256,128,64,32, μg / ml concentration; metronidazole as the control group, respectively, with different concentrations of cinnamic aldehyde and metronidazole liquid Add the liquid medium within the TSB Actinomyces strains, carbon dioxide condition for 24 hours, the microscopic observation of the growth of bacteria smear. 2, cultured periodontal ligament fibroblasts, the subculture of the fourth generation used in the experiment. Within the cultured Actinomyces centrifuged, and the wet bacteria added to an equal volume of n-butanol wall components, the oscillation centrifuged, and the precipitate was freeze-dried. The freezing of the white powder was dissolved in chromatographic buffer, using the octyl Sepharose CL-4B chromatography media as Actinomyces extraction within the LTA. Then different concentrations of LTA (130.9,65.45,32.72,16.36,8.18 mg/m1) and periodontal ligament fibroblasts (density of 5 × 103 cells / m1) co-culture, ELISA was used to detect cell culture supernatant TNF -α levels; cinnamic aldehyde and then formulated 256,128,64,32,16 μg / ml concentration of the liquid, together with the LTA-induced periodontal ligament fibroblasts were cultured for 24 hours, using ELISA cell culture supernatant fluid content of TNF-α. Results: 1 cinnamaldehyde can significantly inhibit the growth of Actinomyces, the minimum inhibitory concentration MIC are 64μg/ml. 2, LTA can induce periodontal ligament fibroblasts to produce TNF-α, to LTA generated when the concentration of 16.36mg/ml up. 3, cinnamaldehyde can inhibit Actinomyces within the LTA-induced periodontal ligament fibroblasts to produce TNF-α, and with the increasing concentration of cinnamic aldehyde, the stronger inhibitory effect. Conclusions: 1 Actinomyces within the LTA can induce periodontal ligament fibroblasts to produce TNF-α. 2, cinnamic aldehyde Actinomyces of internal LTA-induced periodontal ligament fibroblasts produce TNF-α levels are inhibited.
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