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The Expression of TGF-β、IGF-Ⅰ and TNF-α Related to rhBMP-2 Used in Rabbit Posterolateral Lumbar Spinal Fusion Process

Author: WangLeiLei
Tutor: JinGeLe
School: Xinjiang Medical University
Course: Surgery
Keywords: Recombinant human bone morphogenetic protein-2 Transforming growth factor-beta Insulin-like growth factorⅠ Tumor necrosis factor-α real-time PCR
CLC: R687.3
Type: Master's thesis
Year: 2011
Downloads: 3
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Abstract


Objective:To determine the transforming growth factor-beta (TGF-β)、insulin-like growth factorⅠ(IGF-Ⅰ) and tumor necrosis factor-a(TNF-a)expression of the bone fusion masses taken from the rabbits undergone posterolateral lumbar spinal fusion with rhBMP-2/allograft composite, autograft and allograft, and investigate the mechanism of bone formaton. Methods:Sixty adult male New Zealand white rabbits were randomly divided into three groups(autograft group, composite group and allograft group), and each group included 20 rabbits.The rabbits were undergone L5, L6 intertransverse process fussion respectively using autograft, rhBMP-2/allograft composite and allograft.Four rabbits from each group were sacrificed and the bone fussion masses were havested at the 7d,14d,21d,28d and 35d..Determined the TGF-β、ⅠGF-Ⅰand TNF-αexpression by real-time PCR.Results:The expression of TGF-β:At the first 7d after operative, the TGF-βexpression of the composite group was lower than the autogeneous group, but there was no significant difference between them (P>0.05).At the 14d,the TGF-beta mRNA expression of composite group was higher than the autogeneous group,and the difference was statistically significant (P< 0.05).At the 21d, the TGF-p expression of composite group, autogeneous group and allograft group reached the peak, the composite group (3.1163±0.1051) had statistical difference compared to the autogeneous group (2.2349±0.2822)and allograft group(2.2349±0.2822)(P<0.05).At the 28 d and 35d, the TGF-P expression of 3 groups began to decrease, but the expression of composite bone group still had statistical difference compared to the other two groups(P< 0.05).The expressionof IGF-Ⅰ:At the first 7d after operative, the IGF-Ⅰexpression of the composite bone group was lower than the autogeneous bone group, and the difference was statistically significant(P<0.05).But it had higher increment speed than autogeneous bone group.Meanwhile,at the 21d,the expression of composite bone was higher than the autogeneous bone group(P<0.05).At the 28d,the IGF-Ⅰexpression of the three groups reached the peak, and the composite group (4.2720±0.4293) had statistical difference compared to the autogeneous group (2.6645±0.1702)and allograft group (1.6034±0.1704) (P<0.05).At 35d, the IGF-Ⅰexpression of the three groups began to decrease, but theexpression of composite bone group still had statistical difference compared to the other two groups(P< 0.05).The expression of TNF-α:From the first 7d to 28d after operative,there was no significant difference between the composite bone group and autogeneous bone group (P>0.05).While the TNF-a expression of allograft group was higher than the autogeneous bone group and composite bone group.and the difference was statistically significant(P<0.05).At the 35d,the TNF-a expression of the three groups reached the peak, the composite group (1.9714±0.2719) and autogeneous bone group (2.6911±0.1299) had statistical difference compared to the allograft group(3.4902±0.3664)(P<0.05).,there was also no significant difference between the composite bone group and autogeneous bone group. Conclusion:The rhBMP-2/allograft composite is an ideal bone graft materal which chould release rhBMP-2 slowly and constantly.Meanwhile the rhBMP-2 could improve the TGF-βand IGF-Ⅰexpression effectively,and cut the TNF-a expression.

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CLC: > Medicine, health > Surgery > Orthopaedic Surgery ( movement system diseases,orthopedic surgery ) > Orthopedic surgery and surgery > Bone surgery
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