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Oriental Lily 'Emperor Bo \injured tissue and rooting, in order to obtain different auxin lily tissue culture effect studied to determine the best medium formulation to provide technical reference for the production. The results show that: using MS medium containing 0.5 mg / L 6-BA, NAA added has a significant effect in the test concentration range 0.0-0.9 mg / L, NAA added amount of oriental lily bulblets induced bulblets induction number was three times a function, the function of the inflection point of the curve (0.36,75.1), and (0.69,131.97). As can be seen from the test results, in the case without addition of NAA can be smoothly induced bulblets and induction was 13%, indicating to lily scales may contain a certain amount of growth regulating substances; from the shape of the curve, NAA concentration of 0.36-0.69 mg / L, with the increase of the concentration of NAA, bulblets when NAA concentration of 0.15-0.36 mg / L, with the increase of the concentration of NAA, the number of induction bulblets rapid increase; induced increase rate gradually slowed; NAA concentration of 0.69 mg / L, the scales for the highest number of explants bulblets induction; NAA concentrations greater than 0.69 mg / L, with the increase of NAA concentration, The number of bulblets induction gradually reduced. The optimum amount of added NAA (0.69 mg / L) compared with the control, the number of generated bulblets can be increased by 11.7 times. 2,4-D add the amount of leaf callus induction rate Bulbs have a very significant impact in the 2,4-D concentration less than 1 mg / L, the explants can not form a callus; the concentration of 2,4-D quadratic function ,2,4-D concentration callus induction rate between 1.2-3.6 mg / L; When the amount of 2,4-D 3.2mg / L when callus induction rate of 60% of the highest value; With the further increase of the concentration of 2,4-D, the induction rate is slightly lower. The proliferation of cultured bulblets sucrose on bulblet weight gain the greatest impact, followed by 6-BA, again for the NAA; relatively low concentration of sucrose ,6-BA, NAA are conducive to the proliferation of small bulbs; sucrose 30g / L 6-BA1.0mg / L NAA0.5 mg / L for the combination of the best small bulbs proliferation medium. 1/2MS better than 1/3MS rooting effect; high concentration of IAA (2.0mg / L) or low (0.5mg / L) affect the rooting effect to moderate concentration of 1.0mg / L effect; 1 / 2MS IAA1.0mg / L IBA0.2mg / L sucrose 30g / L is ideal rooting medium, rooting rate was 95.8%. Lily detoxification study, study more direct shoot tip culture combined effect of heat treatment of detoxification shoot tip culture better, shoot tip size 0.3-0.6mm suitable detoxification rate of up to 55%. Also induction of aerial bulblets culture reproduction preliminary study, MS medium spray to develop scales differentiation bulblets and rooting effect best, scaly average differentiation 3.8 bulblets average rooting number 6.8. May improve the survival rate of plantlets were transplanted Plantlet Transplanting a new idea.
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