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In this paper, Grifola frondosa (Grifola frondosa) for the experimental material, the use of electron beam irradiation, spontaneous atmosphere (MAP) combined refrigeration and chemical methods of preservation solution to the preservation, study the fruiting body postharvest storage during weightlessness, the quality of the sensory related indicators of physiological and biochemical changes in enzyme activity (SOD, CAT, protease), MDA content, soluble protein, total sugar and reducing sugar revealed fruiting body aging and storability, and explore its suitability storage and preservation methods, provide a theoretical basis for large-scale cultivation of Grifola frondosa mining and storage. This paper L 16 (4 5 ) orthogonal experimental design a the Grifola frondosa variety of electron beam irradiation, MAP and cold storage 9008 treatment combinations, study sub- entity postharvest physiological quality during storage as well as the effect of storage and preservation, the relative humidity of the storage are (70 ± 1)%. Analysis can be seen from the sensory evaluation, in addition to 1.4kGy, PVC, and 10 ° C treatment group, all treatment groups than the control group (fruiting bodies with PVC film packaging, storage at 4 ℃ after irradiation). 1 ° C storage conditions under the four treatment groups, the 1.8kGy and OPP handling conditions of 10 ° C group and at 4 ℃ 1.4kGy, the OPP, 1.8kGy, PP and 2.2kGy of OPP treated at least up to a week to extend the preservation time . The orthogonal analysis showed: the 0.8kGy, PE film, and 4 ° C (P <0.05) treatment group significantly to maintain post-harvest quality of Grifola frondosa fruiting bodies, has higher antioxidant enzyme activity and total sugar content of MDA and soluble protein content lower storage early low reducing sugar content; impact Grifola frondosa mining Physiology and Preservation effect of the main factors is the radiation dose, and the secondary factor is packaging film, again for temperature (P <0.05). Other conditions constant, the higher the radiation dose (≥ 1.8 kGy) or higher storage temperature (10 ℃), the greater the impact on the physiology and biochemistry of Grifola frondosa fruiting bodies, the more unfavorable to its storage and preservation. PVC film is not conducive to the storage and preservation of the fruiting body. Frondosa varieties 151 material, respectively, with 0.5% (w / v) chitosan, 1.0% (w / v) chitosan, 2.0% (w / v) chitosan, 0.5? Cl < sub> 2 (w / v) 1.0% (w / v) chitosan, 0.5% (w / v) of CaCl 2 processing, and does not handle as a control, at a temperature 4 ℃, relative humidity of 65 to 70% of the storage conditions, and to explore its postharvest physiology and biochemistry and Storage effect. Experimental results show that: 1.0% chitosan treated with the best preservation effect, the lower fruiting bodies within the MDA, soluble protein and reducing sugar content, with a higher level of CAT activity. 1.0% and 2.0% chitosan treated fruiting bodies within higher SOD activity, weight loss rate is low. 2.0% chitosan treated fruiting bodies of protease activity. 0.5? Total sugar content of Cl 2 treatment group fruiting bodies showed a higher level. Good 0.5? Cl 2 1.0% chitosan mixed treatment group of the fruiting body of physiological and biochemical impact Preservation Effect not be used alone or 1.0% chitosan treatment group. Antioxidant enzyme activity during storage, after several preservative treated fruiting bodies higher than that of the control, the protease activity is lower than that of the control, play a different role in the enzyme activity is maintained at favorable levels of storage and preservation, can delay the aging body . Frondosa pharmacologically active polysaccharide is a main component and the amount directly affect the value of commodities. Polysaccharide content of 0.5? Cl 2 treatment group compared with the other groups, but for the preservation of edible fungus needs from an integrated quality analysis, the treatment group has little effect on other physiological and biochemical indices preservation effect. poor.
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