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Antifungal Substance Produced by Bacillus Subtilis SH7 and Its Biological Control Effect Against Tobacco Bacterial Wilt

Author: ZhangXiuYu
Tutor: KongFanYu
School: Chinese Academy of Agricultural Sciences
Course: Plant Pathology
Keywords: SH7 Bacillus subtilis Antimicrobial substances Purification Feature Biocontrol
CLC: S435.72
Type: Master's thesis
Year: 2009
Downloads: 300
Quote: 4
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Abstract


Through this paper, Bacillus subtilis SH7 Purification and Characterization of antimicrobial substances for research and Biocontrol of tobacco bacterial wilt research proven Bacillus subtilis strain producing antimicrobial proteins SH7 optimum fermentation conditions , characteristics and antibacterial protein biocontrol of tobacco bacterial wilt effect . Antibacterial spectrum test showed that , SH7 variety of plant pathogenic fungi , bacteria have a strong inhibitory effect . Is produced by bacteria and the development and utilization of the active material to provide a basis for the biological control of plant diseases can also provide biological research base , the main results are as follows : 1. After shaking flask experiments were screened out SH7 producing Bacillus subtilis antimicrobial activity substances optimal culture conditions namely: best medium for NA liquid medium , the optimum fermentation conditions were : pH6.0, 26 ℃, liquid volume 100mL, 170 r / min shaking 96h, this time on the tobacco Ralstonia maximum antibacterial activity of bacteria , which produce active substances most. (2) Determination of antibacterial spectrum showed , SH7 strains produce antimicrobial proteins on a variety of plant pathogenic fungi, bacteria have a strong inhibitory effect . The antibacterial protein has better thermal stability , at 100 ℃, 121 ℃ treatment 20min, the antibacterial activity remains at 83.1% , 68.0% . Antibacterial protein in neutral and alkaline environment is relatively stable , at pH9.0, pH10.0 remain when the antibacterial activity of 86 %, 88% . Proteinase K, pepsin crude extract almost no effect , antibacterial protein having amylase activity . SH7 strains secrete antimicrobial proteins can reduce tobacco bacterial wilt amount of secreted extracellular polysaccharide . 3 by ammonium sulfate fractionation , DEAE Sepharose Fast Flow ion- exchange chromatography and gel filtration on SephadexG-75 of 30% saturation of ammonium sulfate precipitation of the protein purified by a kind of antibacterial proteins , polypropylene polyacrylamide gel electrophoresis for molecular weight determination of the inhibitory protein molecular weight of about 33.6KDa. 4 in a greenhouse pot experiment results show that the antimicrobial protein crude extracts from the effects of tobacco bacterial wilt 70.8% ; demonstration in the field trials, biocontrol agents SH7 and biocontrol agents SH7 granules with 72% A cream spirit . MZ WP mixed fertilizer mixing occurs on tobacco bacterial wilt field efficiency were 30.36% and 46.86% , better than the single application of 72% metalaxyl . MZ WP control effect .

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CLC: > Agricultural Sciences > Plant Protection > Pest and Disease Control > Crop pests and diseases and their prevention > Economic crop pests and diseases > Tobacco pests and diseases
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