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Objective: In this study, by observing the Schwann cell growth in different aperture silk fibroin bracket on the preliminary assessment fibroin scaffold different morphologies of cell growth . Materials and methods : ( 1 ) preparation of different pore size porous silk fibroin material . Respectively: macroporous materials (pore diameter of about 50 to 60μm) , pore material (pore size 10 to 20μm) , in groups of three samples . 2 selection Schwann cells immortalized cell R3 [ 33 - 10ras3 ] as seed cells , Schwann cells at 37 ° C , 5% CO 2 incubated inside the train , when the cells in the bottom of culture flask forming a dense monolayer can be digested cells and inoculation experiment , the use of 5 ? S adjustment after digestion of cell concentration , the concentration of 1 × 10 to 6 / ml of the Schwann cell suspension was planted in the different the morphology of porous silk fibroin material surface . Schwann cells - silk fibroin composite materials after one week of training , scanning electron microscopy cell morphology , proliferation and other circumstances . Results: the different aperture material visible on the surface of Schwann cell growth is variable . The bracket of the small pore material , the cell performance of a specific bipolar morphology between cells or arranged in parallel, or both connected to the chains of cells ; bracket of a large pore size material , the cell density is high , but the cells were mostly spherical single dispersed in the surface of the porous scaffold , or rendering the cells were bipolar sample agglomerated clusters of grapes - like gathered at the bottom of the hole and is not extended dual polar form , only a very small amount of growth in the crest between holes . Conclusion : the silk fibroin bracket the morphology of Schwann cell adhesion , growth has some influence . Schwann cells seem to have a tendency to grow on the ridge between the hole and the hole . The future improvement of the scaffold morphology , stent designed to adapt to the growth of Schwann cells , which provide some reference .
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