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The Expression of PCNA and TNF-α on Liver Regeneration after Partial Hepatic Resection under Different Hepatic Inflow Occlusion Methods in Rats

Author: WangBingJie
Tutor: JuXinHua
School: China Medical University
Course: Surgery
Keywords: Liver regeneration Hepatectomy PCNA Hepatic inflow occlusion TNF-α
CLC: R657.3
Type: Master's thesis
Year: 2009
Downloads: 41
Quote: 0
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Abstract


The aim of the present study is the law through three different hepatic artery occlusion rats underwent 70% hepatectomy by Higgins method to induce liver regeneration , compare three methods of liver regeneration . Method 1 , an animal model using rat liver anatomical characteristics , respectively pringle law , the selective retention of non - disease side hepatic artery hepatic inflow occlusion law and semi hepatic vascular occlusion , and then resected liver lobe and right hepatic leaves (about 70%) of the liver to induce liver regeneration . 2 , the evaluation of liver regeneration by means of the general form of the above model observed at different time points in rat liver ; liver weight ratio was measured at each time point after surgery ; the HE staining count nuclear division ; immunohistochemical method detected six hours after surgery , 12 hours , 24 hours relative to point PCNA labeling index was measured after 6 hours ; RIA , 12 hours, 24 hours serum TNF-α indicators . 1 , liver weight and weight of liver weight body weight ratio in each experimental group than with time gradually increased; one day liver body weight among the groups no significant difference ; 4 days and after 7 days WH group and HH group liver heavy weight ratio is greater than the PR group , the WH group and HH group difference was not significant . 2 , the mitotic count PR group mitotic phase in one day after gradually increasing to seven days , the WH group and HH group 1 day already increased 4 - day split phase up to 7 days has dropped . 1 day and after four days the WH group and HH group than the PR group , WH group and HH group difference was not significant ; 7 days after each group no difference between . 3, PCNA expression of PCNA expression in the postoperative the 6h group and normal group differences in postoperative 12h , WH group and HH group PCNA expression ; 24 hours of PCNA expression , WH group and HH group and PR group differences. Each time point between the WH and HH the difference was not significant . 4 , serum TNF-α level in each group of TNF- α level was elevated at 6 hours after the PR group increased most significantly , has been gradually reduced in the 12 hours of TNF-α . Of conclusions 1.WH group and HH group, PCNA and TNF-α was no significant difference may be no significant difference between the two groups of liver regeneration . 2.WH significant difference between group and PR group , PCNA and TNF-α , may be significantly different between the two groups of liver regeneration .

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CLC: > Medicine, health > Surgery > Of surgery > Abdominal surgery > Liver and liver tube
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