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Neuroprotective Effects of Ac.Yvad.Cmk on Experimental Spinal Cord Injury in Rats

Author: KongRanRan
Tutor: TuGuanJun
School: China Medical University
Course: Surgery
Keywords: AC.YVAD.CMK Spinal cord injury Apoptosis IL-1β
CLC: R651.2
Type: Master's thesis
Year: 2009
Downloads: 47
Quote: 0
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Abstract


Purpose of spinal cord injury pathological processes , including primary injury and secondary injury , the damage caused by the secondary injury than the primary injury , the treatment of human spinal cord injury secondary to neurological dysfunction is still a very difficult problem , nerve cells apoptosis play an important role in secondary injury , and to observe the role of Caspase-1 inhibitors AC.YVAD.CMK inhibit neuronal apoptosis in rats with acute spinal cord injury and inflammatory response . Method healthy adult SD rats ( male or female ) 50 ( provided by the China Medical University Animal ) , weighing 250 ± 25g . Were randomly divided into three groups : sham-operated group , SCI group , AC.YVAD.CMK treatment group . The sham group ( n = 10 ) , the SCI the group and AC.YVAD.CMK treatment group according to the postoperative time points 24h and 72h group , n = 10 ; 24h and 72h in rats neurological function after spinal cord injury , spinal cord injury site line pathomorphology , IL-1β immunohistochemical expression and neural cell apoptosis observed . The results can be seen in the light microscope injured spinal cord tissue applications AC.YVAD.CMK relieve the edema and hemorrhage were focal decrease in neutrophil and macrophage infiltration , cytoplasmic vacuolization and nuclear pyknosis phenomenon is reduced ; white matter axons edema , vacuolar degeneration lighter . The number of TUNEL-positive apoptotic cells between the the SCI group AC.YVAD.CMK treatment group statistically significant difference ( P lt; 0.05 ) . IL-1β expression was increased after SCI , IL-1β involved in the secondary injury process after spinal cord injury , the positive expression of IL-1β AC.YVAD.CMK treatment group below the SCI group ( P lt; 0.05 ) , 72h two Tarlov score of spinal cord motor function between the groups there are significant differences (P lt; 0.05). Conclusion AC.YVAD.CMK inhibit the apoptotic response and inflammation, and neuroprotective effects of acute spinal cord injury in rats . Through this experiment provides a reference for the clinical treatment of spinal cord injury .

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CLC: > Medicine, health > Surgery > Of surgery > Head and Neurosurgery > Spinal cord
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