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Experimental Neuroimmunomodulation Study on Treating Aging Rats by Electro-acupuncture on Guanyuan and Zusanli

Author: LiJia
Tutor: WangHua
School: Hubei College of Traditional Chinese Medicine
Course: Acupuncture and Massage
Keywords: Subacute aging / experimental Strong points Neuropeptide Y Interleukin- 6
CLC: R245
Type: Master's thesis
Year: 2009
Downloads: 88
Quote: 1
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Abstract


Purpose in order to address the burden of aging in the 21st century, the experiments using a variety of modern research techniques, D-galactose induced aging rats as subjects, the strong points of acupuncture on the central nervous system of immune regulation, and compare differences of the two stimuli parameter effect acupuncture acupuncture clinical research exploring the best methods, and provide experimental basis for further in-depth research. Methods March 40 old SD rats, the random number table, select 10 for the normal control group (A), cervical subcutaneous injection of normal saline 125mg/kg · d, 40 days, another 30 line D-galactose 125mg / kg · d the neck subcutaneous injection 40 days replicative senescence model rat water maze test screening model rats to reach the platform significantly longer time, into the blind end of the increased frequency of successful modeling. Modeling successful 30 rats were randomly assigned into aging model group (group B, 10), the conventional electro-acupuncture group (C, 10), strong stimulation EA group (D, 10) . Normal control group, aging model group without treatment; C group rats wearing homemade rat clothing, take off, referring to the 1992 Chinese acupuncture the experimental acupuncture research \The Hua Tuo brand, 15mm × Φ0.32mm, acupuncture needle acupuncture, then G6805-Ⅱ type electro-acupuncture treatment instrument then negative, Guan Yuan, Zusanli then positive, bilateral Zusanli every other day, alternating use, the choice of continuous-wave, frequency 2Hz strength of 1mA . Energized for 30 minutes at a time, once a day, six times a week, a total of 4 weeks of treatment, and drawn the day after the last treatment. Group D of electric acupuncture and drawn with group C, but the needling time was 5 minutes, the intensity of 4.5mA. The end of the treatment, eyeball blood, serum interleukin-6 (IL-6) content determined by radioimmunoassay. After rats were killed, and the brain tissue, in situ hybridization to detect neuropeptide Y (NPY mRNA) gene expression levels, immunohistochemistry SABC method of rat interleukin 6 receptor (IL-6R) expression levels, the use of image analysis systems analysis detected NPYmRNA the immunoreactive material expression of the IL-6R, and data for statistical analysis. Results 1. Compared with the normal control group, induced aging rat hypothalamic paraventricular nucleus NPY mRNA levels decreased serum IL-6, IL-6R levels in the hypothalamic paraventricular nucleus statistical analysis treatment difference was significant (P <0.05) compared with the aging model group, two intensity EA treated rats hypothalamic paraventricular nucleus NPYmRNA elevated serum levels of IL-6 in the hypothalamic paraventricular nuclear IL-6R levels were reduced, statistical analysis and processing The difference was statistically significant (P <0.05) 3. conventional electro-acupuncture group (1mA) and strong stimulation of the EA group (4.5mA) two sets of comparative statistical analysis of treatment differences were significant (P <0.05). Conclusion 1. Acupuncture strong points significantly improve the the aging rats reduced NPYmRNA expression, reduce aging rats too high levels of IL-6R and peripheral blood IL-6 expression content, which may be the acupuncture stimulated rat central nervous system and to enhance endocrine immunomodulatory effects. Conventional electro-acupuncture with strong stimulation efficacy of electro-acupuncture, strong stimulation electroacupuncture current stimulus intensity, although higher tolerance, but more effective than conventional electric needle, and the time is short, you can provide new acupuncture treatment for future ideas. This topic to explain acupuncture strong points to improve aging the state neuroimmunological role mechanism provides a scientific experimental basis, reflecting reinforce righteousness oriented thinking advantage.

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