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Research and Application of Mimic Enzyme Biosensors and Cell Biosensors

Author: XuXing
Tutor: ZhangAiDong；TuHaiYang
School: Central China Normal University
Course: Pesticides
Keywords: Biosensor Biomimetic Cell biosensors Herbicide Non-specific adsorption
CLC: TP212.3
Type: Master's thesis
Year: 2009
Downloads: 101
Quote: 0
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Abstract


This thesis work mainly includes the following aspects: 1) superoxide dismutase intends enzyme the CHH-Cu self-assembly as a biosensor for superoxide anion detection; 2) 4 - (mercaptobutyl-) triglycol Synthesis Its non-specific adsorption of protein; 3) Synechococcus suspension cells fast chlorophyll fluorescence induction detection herbicide;) Synechococcus cells biosensor based on gold signal amplification, the herbicide detection. The specific contents are as follows: 1) the tripeptide (CHH) modified by self-assembly technology to the gold electrode surface, and then to form complexes with copper metal ion coordination that Cu / CHH / Au superoxide dismutase Biomimetic biosensor. These modified electrodes for electrochemical characterization of the contact angle, XPS, and the determination of the self-assembly system modified electrode disproportionation of the superoxide anion. Superoxide anion concentration to between 9.36 and 65μM Cu / CHH / Au under the conditions of 350mV and-150mV sensitivity were 1.042x10 -4 AM -1 and 2.36 × 10 -4 AM -1 . 2) triethylene glycol and 1,4-dibromobutane as raw material, the synthesis of the target compounds with self-assembly features 4 - (mercaptobutyl-) TEG. The molecular structure of the target compound 1 H NMR, GC-MS and IR confirmed. Assembling the target compound to the electrode surface by the electrochemical characterization and surface plasmon resonance (SPR) to study the self-assembled film of bovine serum albumin (BSA), non-specific adsorption of the compound. 3) Synechococcus cells cultured and uniform cell suspension system, measured by the fast chlorophyll fluorescence analysis the Synechococcus suspension cells atrazine inhibition of photosynthesis. The role of Atrazine concentration for 10 -5 mol / L, 10 -6 mol / L the cells in Photosystem Ⅱ Q A after electron transfer is substantially completely suppressed, when the action concentration is reduced to 10 -7 mol / L, corresponding to inhibition decreased, there does not completely inhibit the phenomenon, and the concentration was reduced to 10 -10 mol / L, substantially not suppressed. 4) a mixture of chitosan and gold uniform, as a fixed media Synechococcus cells embedded immobilized on the gold electrode by electrochemical methods to study atrazine chlorophyll photosynthesis system Ⅱ (Ps Ⅱ) electronic transmission, gold nearly tripled improve the electrochemical signal. The results show that the optical current suppressing half atrazine effect concentration of approximately 5 × 10 -8 mol / L, and the atrazine concentration range of 1 × 10 -6 to 1 × 10 -8 mol / L when the current size and the corresponding concentration of atrazine logarithmic value showed a good linear relationship.

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CLC: > Industrial Technology > Automation technology,computer technology > Automation technology and equipment > Automation components,parts > Transmitter ( converter),the sensor > Biological sensors,medical sensors
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