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Because eukaryotic cells DNA replication, transcription and expression of the protein translation become one of the most important biological functions in the cells within the cell partition and biological macromolecules such as proteins and nucleic acids cross karyotheca transporter . importin-β family protein substrate mediated nucleocytoplasmic transport is one of the main mechanisms . importin 13 (imp13) is one of the members of this family , is currently the only known mammalian cells bidirectional transporter substrate proteins protein . Studies have found UBC9, Rbm8, Pax6, NF-YB/NF-YC, GR, myopodin, negative cofactor 2, CHRAC-15/17, p12/CHRAC-17 and eIF -1A of importin 13 bottom material . These substrates are mostly related to the development , importin 13 may have important biological functions in the body development . In 2004 , Tao et al found that of importin 13 stranded after missing the N-terminal in the cytoplasm ; 2006 and 2008 , Tao found that C-imp13 negative regulation of importin 13 transporter function ; 2006 , Yasuka such as found in mouse testis of importin 13 another transcript (TS-imp13), it contains exon of importin 13 of the sixth intron of 75 bp and after the C-terminal 14 outside . They speculate the TS-imp13 may play a negative regulatory role of importin 13 transport function . Recently , Giagtzoglou has etc. found , importin 13 can be adjusted the Drosophila neuromuscular connections neurotransmitter release , deletion importin 13 cause the decline in visual ability of Drosophila and death in the larval stage , instructions of importin 13 of the nerve function and the development of the body important functions. In order to study of importin 13 during development , especially neurodevelopmental process , this study was constructed the C-imp13 (aa450-963) transgenic mice TRE- C - imp13 N-terminal deletion and according to the principle of the Tet-on system , real-time regulation of its expression. Born 100 animals , Genotyping detected 27 to contain TRE-C-imp13 transgenic offspring ( 27 founders ) ; RT-PCR and real-time PCR method for screening out the four expression of C-imp13 founder, for future research importin 13 provides an important animal model for the role of developmental processes .
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