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Distribution of Calcium, Polysaccharides and Lipids in Developing Anthers of Ipomoea Cairica (L.) Sweet

Author: LiuHuanHuan
Tutor: TianHuiQiao
School: Xiamen University
Course: Developmental Biology
Keywords: Cairica Anther Calcium Polysaccharide Lipids
CLC: Q945.4
Type: Master's thesis
Year: 2009
Downloads: 157
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Abstract

This experiment using coke antimoniate positioning calcium precipitation technique, using the staining technology positioning polysaccharide and lipid, by optical microscopy, transmission electron microscopy observation, calcium for anther development cairica (Ipomoea cairica (L.) Sweet) ions, polysaccharides, lipids distribution characteristics of a more detailed study. The results are as follows: (1) the use of ultra-thin sections and transmission electron microscopy techniques, found, cairica anther of the Anthers of cairica in internal structure observed more special about two points: ① medicine wall has two middle within the middle in the degradation of extracellular pollen late; ② tapetum shaped tapetum, has a dual origin. By anther wall cells during the formation of the sporogenous anther wall tapetum, microspore mother cell stage formation anther tapetum by antherlobe cells. Two tapetum was no significant difference in terms of morphology, disintegration time. In addition, by transmission electron microscopy observation, the the pollen wall cairica development process is summarized as follows: tetrad pollen exine began to take shape. To the the microspore late intine development. At this point, the the the pollen exine obvious District into overlay-based tablets stick layer and endexine. The extracellular pollen period, pollen inside and outside wall to continue development, pollen inclusions gradually increased, a significant increase in pollen volume. (2) the use of coke antimonate precipitation technique and transmission electron microscopy techniques to observe anther development of cairica calcium distribution, the results show: the sporogenous cell stage, calcium particles are mainly distributed in the cytoplasm of sporogenous cells. Microspore mother cell stage, the calcium particles concentrated in the callose wall. The tetrad microspores calcium particles distribution callose wall and small spores at the outer wall primordium. After that the microspore developmental process, calcium particles are mainly distributed in the vacuole formation at small spore cells and vacuole. In addition, in the microspore wall of the outer wall of the outer layer and the inner wall of the distribution have a greater calcium particles was lamellar endexine tiny calcium particles and fewer in number, and finally disappeared. The two cellular pollen intine calcium particles disappear only in the outer layer of pollen exine distribution. According to the above results suggest that the main role of the calcium ion in pollen development: to participate in the formation and dissolution of the callose wall, to participate in the formation of large vacuoles and pollen wall. In anther wall tissue: microspore mother cell stage before anther wall cells more calcium particles. Epidermal cells, most of the calcium particles distributed in the vacuole, endothecium and middle cell calcium particles are mainly distributed in the vacuole edge, cytoplasm and cell wall. To the tetrad stage, the epidermis, drug indoor wall and middle calcium particles decreased slightly and continue until late microspore development. The two extracellular pollen is formed, the calcium precipitation is further reduced. : Pre-dissolution in the tapetum cells, the distribution of calcium particles in the cell wall edge; after the disintegration of the early microspore, calcium particles are distributed in the cell remnants, and formed by the tapetum the Ubbelohde body surface of the calcium particles. Connective tissue and vascular xylem cell wall, vacuole edge or vacuole always calcium particle size distribution, but significantly reduced in the two cellular pollen period. These findings suggest that: in the early anther development, the plants calcium ions by connective tissue is first transported to anther wall cells. Childhood microspore mother cell to the microspore development late anther wall cells calcium transporters to the drug chamber. At this point, the the Ubbelohde body formed by the tapetum cells, the calcium transfer to the disintegration of the callose wall and microspore wall. After a period of two cellular pollen, due to reduced demand drug indoor pollen of calcium, more calcium particles concentrated nutrients in the pollen exine drug chamber cavity. (3) the use of semi-thin sections and staining techniques anther development cairica polysaccharides and lipids distribution observed: sporogenous cells more polysaccharide particles, but no lipid droplets. Microspore mother cell meiosis, intracellular polysaccharide granules disappear, small lipid droplets. Small meiosis, intracellular lipid droplets decreased, but the re-emergence of the polysaccharide particles. Until microspore later developmental stages in the formation of large vacuoles, lipid droplets began to increase. Microspore mitosis, polysaccharide and lipid droplets of two extracellular pollen accumulated gradually increased, polysaccharide and lipid droplets of substance in mature pollen. Sporogenous cell stage, the epidermal cells of the anther wall has been widely polysaccharide particles, the mother cell stage endothecium cells also appear polysaccharide particles. Thereafter, the epidermis and drug chamber wall cells has been distributed more of the polysaccharide particles and less lipid droplets. More lipid droplets within the intermediate cells from the mother cell stage to the tetrad. Tapetal cells before the disintegration of polysaccharide particles, and no obvious distribution of lipid droplets. The microspore early after the disintegration of tapetal into the lipids accumulated in the medicine room cavity. Cellular pollen periods within the intermediate cells degraded its cytoplasmic material into the same lot of lipids. According to the distribution the anthers of polysaccharide and lipid droplets, polysaccharides and lipids role in pollen development can be divided into two stages: before two cellular pollen, the small spores within the polysaccharide may be used for the synthesis of callose wall and intine lipid droplets and vacuolar membrane and exine synthesis. After two cellular pollen of pollen polysaccharide and lipid droplets as nutrients large reserves after germ cell development, pollen tube germination process raw materials and power. Anther wall cells nutrients transport process can be divided into two stages, tapetal cells degenerate before absorption by the tapetum cells, the transporter of nutrients into the medicine room; tapetal cells degenerate, from within the intermediate cells uptake transporters. Finally, within the middle cell degradation products as a source of nutrients in the two cellular pollen.

Distribution of Calcium, Polysaccharides and Lipids in Developing Anthers of Ipomoea Cairica (L.) Sweet

Abstract

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