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Seven members of the signal transduction and activator of transcription (STAT) family have been found as a transcription factor , a variety of cytokines , hormones and growth factors can influence signal transduction of its activity , thereby regulating the expression of downstream target genes , influence and change the cell functions, such as cell proliferation , differentiation , apoptosis , transformation, is aware of the immune response , inflammation, tumor closely related . In 7 members , STAT3 was studied most , and gene knockout studies have found that it is the only members of this family knockout gene cause embryonic lethality Description The gene has an important role in the formation and development in self . Because of its importance , it is in the ascendant , especially what proteins interact and regulate its transcriptional activity is a hot spot in the study To this end, we used the yeast two-hybrid technology , STAT3 decoy , screening adult liver library its interacting proteins , the results of our screen to a new prey sorcin . Few studies about Sorcin known that it is a soluble pentyl -EF hand -type Ca to 2 binding protein , the molecular weight size of 22 kDa , was originally found in the multi- drug resistance cells , it may be associated with resistance ; In addition , the report displays the associated Sorcin with multiple myeloma , lymphoma , breast cancer and other cancers . Because there have been reports STAT3 tumor and drug -related , so we continue to study further whether the interaction between the two . A result , we successfully amplified by PCR the Sorcin gene was successfully constructed with Myc-tagged Sorcin expression vector with GST-tag and Flag label STAT3 carrier , Western - blot detection of expression of the three , and then by GST - pulldown experiments and Co- the IP the experiment further evidence of the of STAT3 and Sorcin the interaction Finally, we take advantage of the luciferase reporter gene experimental study found Sorcin regulation of transcriptional activity of STAT3 , and this increase in the role of a dose - dependent effect . In conclusion, we found that for the first time the important transcription factor STAT3 interacting protein sorcin , and preliminary evidence of interaction they are physically and functionally laid the foundation for further study of the interaction between the two , may also be to study the anti- the drug mechanism provides a new clue .
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