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Black fungus polysaccharides, oligosaccharides preparation and application of

Author: JiangLiLi
Tutor: ZhangYanLong
School: Heilongjiang University
Course: Microbiology
Keywords: Auricularia auricula polysaccharide Auricularia auricula oligosaccharide Marine bacteria Degeneration enzyme Biological activity
CLC: TQ461
Type: Master's thesis
Year: 2009
Downloads: 339
Quote: 1
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Abstract


Auricularia auricula is the edible fungus for medical purposes and includes many polysaccharides. Auricularia auricula polysaccharides degenerated by specific enzymes from marine bacteria produced Auricularia auricula oligosaccharide with biological activity. The Auricularia auricula polysaccharides and oligosaccharides have many kinds of biological functions, for example, falling down blood sugar, anti-thrombus, anti-tumor and enhancing immunity.This thesis was carried on the optimal design to the process of extraction of Auricularia auricula polysaccharide, under the condition of pH 5.0, the water bath temperature 100℃, water time 2h and the feeding measures 2g. The highest rate of polysaccharide production was 13.64%. Simultaneously select sea microorganism which had the function of reducing Auricularia auricula polysaccharide biological activity, and appraise the shape, the physiological biochemistry characteristic of the fungus. After this, the strain was Aeromonas and identification of the enzyme activity was carried out. The results showed that the enzyme activity was strongest when the temperature 30℃, pH6.5, substrate 1.0mL, enzyme 0.5mL. Auricularia auricula polysaccharide from the paper had the anti-hypertension activity. The isolated enzymolysate was determind to be Auricularia auricula oligosaccharides using the infrared spectroscopy method.So far, no one studies the preparation and effectiveness of Auricularia auricula oligosaccharides at home and abroad. A lot of its effects are better than Auricularia auricula polysaccharides. Auricularia auricula oligosaccharides have great application prospects and play a huge role in economic development and advances in medical and health research.

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