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Characterization of Focal Adhesion Kinase and ADP Ribosylation Factor from Marsupenaeus Japonicus

Author: ZhangMingChang
Tutor: XuZuo
School: Xiamen University
Course: Biochemistry and Molecular Biology
Keywords: FAK Arf WSSV
CLC: S917.4
Type: Master's thesis
Year: 2009
Downloads: 42
Quote: 0
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Abstract


The research papers japonicus focal adhesion kinase (MjFAK) and small G protein ADP ribosylation factor (MjArf4) conducted a preliminary study , and at the same time to establish an effective phage display library screening methods to find protein interactions with them , to lay the foundation for future research MjFAK and small G-protein signal transduction mechanisms in WSSV infection . FAK is a class of non-receptor tyrosine kinase , in higher organisms have been widely reported involved in cell adhesion , anti-apoptotic , actin reorganization, and tumorigenesis . Us for the first time in crustaceans cloned into FAK. Sequence analysis showed that MjFAK the middle containing conservative PTK domain and the C-terminal FAT domain . MjFAK of Tyr399, Tyr579 been shown to occur phosphorylation . In addition , in the early the WSSV viral infection japonicus , raised its of blood cells MjFAK protein expression levels , caused by rising levels MjFAK (pY399) and MjFAK (pY579) . Study of infected blood cells the Fn (Fibronectin) adhesion also found blood cell adhesion on Fn with MjFAK phosphorylation levels . Therefore , we infer the shrimp blood cell MjFAK all play an important role in the viral invasion and host defense . The Arfs belongs to the Ras family of small G protein from Japan capsule the shrimp cloned belongs to Class Ⅱ the Arf ( named MjArf4 ) . The study found 72 h after infection of WSSV MjArf4 expression levels significantly increased, prompted MjArf4 virus infection closely linked . Further studies showed that the promoter in MjArf4 at -18 bp containing a conservative CRE (cAMP Response element) , the analysis of promoter activity indicates that the CRE in transcriptional regulation might have a role in inhibit MjArf4 transcription . The MjArf4 transfection into HighFive insect cells found , MjArf4 may be located in the Golgi active mutation analysis showed distribution of MjArf4 is GTP - dependent . In vitro virus phagocytosis was found that BFA does not affect blood cell phagocytosis of virus . These results suggest that MjArf4 late to play a significant role in WSSV infection .

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CLC: > Agricultural Sciences > Aquaculture, fisheries > Aquatic basic science > Aquatic Biology > Aquatic Zoology
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