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Resources of mainland China macaque species are extremely rare and with a decreasing trend in the field investigation and protection of law enforcement work Macaca suspected samples tend to pollution, corruption, incomplete (such as hair, bone, hoof, organ, muscle fiber etc.), or a negligible amount of the subject material, and difficult to check identification. Macaca wild animals in order to protect the more powerful, to crack down on illegal smuggling and hunting, is very meaningful to the establishment of the Chinese mainland macaque species identification techniques of molecular biology. Test: This test is a non-invasive method, from mainland China four macaques case of species Fecal extracted DNA from Genbank download some common animals, Macaca animal species and the same family is similar to the case of similar Branch with the Head cyt b gene sequence of the animals, the use of software design and synthesis Macaca universal primers (Ms / Ma), the pig-tailed monkey specific primers 1 pairs (Ts / Ta), macaque-specific primers (Ys / Ya), short-tailed Monkeys two pairs of specific primers (Ds1/Da1, Ds2/Da2), the specific primers assamensis 2 on (Xs1/Xa1 Xs2/Xa2), and through a large number of tests to verify their specificity, stability and sensitivity. The results show that: the universal primers Ma, Ms species of macaque DNA specific, only specific amplification macaque species DNA, the DNA of some common animals and animals near the source could not be amplified; several other pairs of primers with good The specificity and stability. Especially sensitive test, measuring out of each of the primers can be detected the lowest concentration of the species required. Test: Primer Express software design and synthesis Taqman probe: a pig-tailed monkey (Ts / Ta), macaque 3 the (Ms0/Ma0 Ms1/Ma1 Ms2/Ma2) macaques 1 (Ds1/Da1 ), an Assamese macaque (Xs1/Xa1), verification by a large number of tests, results show that: macaque probe (Ms0/Ma0), pig-tailed monkey probe (Ts / Ta) and macaques (Ds1/Da1) probe good specificity, can be distinguished from other Macaca area and mainland China. Macaca mulatta in probe (Ms1/Ma1, Ms2/Ma2) can first monkeys, macaques and the bear monkey, pig-tailed monkey area separate the reuse macaques probe (Ds1/Da1) monkeys and macaques can. The trial was the first to use a step by step identification method, this has reference significance for other wildlife identification. Test: samples from a variety of trace: macaques hair, muscle, the macaques hair, blood in the extraction of DNA, to do with the DNA template obtained from fecal samples, the consistency of the test results. The results show that: the DNA obtained from the hair, muscle, blood consistent with the DNA test results obtained from fecal samples. Indicating that the method is stable and reliable, so as to achieve the purpose of identification of trace Organization Macaca. Test: run brightest purpose agarose gel electrophoresis bands Gel Extraction and sequenced, to see whether the result is the target band. The results show that the sequence consistent sequencing resultant sequences and published on the web, is the desired purpose of the strip. Design sequencing primers also been measured four Macaca species Cytb sequence to extract scientific basis for future research. The test to establish four kinds of animals in mainland China Macaca molecular biology inspection and laboratory confirmed. Macaca animal residues encountered in the field survey and some of its inspection of the trace samples to provide a convenient, sensitive, reliable means of detection.
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