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Our country is a a sericulture big country , has a long history of sericulture , silkworm excrement as a byproduct of the sericulture industry , resource-rich , but it has not been a very good use of them , mainly due to more dispersed and difficult to collect the use of . Silkworm excrement is rich in chlorophyll , can reach 0.8 to 1.2 percent , chlorophyll is the natural pigment one of the most widely distributed on Earth exists in green plants , play an important role in the photosynthesis of plants or microorganisms . , Chlorophyll and chlorophyll zinc sodium salt prospects , better adapted to the needs of natural additives , chlorophyll and preparation of sodium zinc chlorophyllin extracted from silkworm excrement , not only to increase the added value of the silkworm excrement , also provides for the comprehensive utilization of silkworm excrement economic assurance and social benefits. The trial of the 07 spring and autumn and 08 of 28 samples collected for determination of chlorophyll and conventional ingredients obtained : silkworm excrement in the chlorophyll content of 0.2 to 0.9% ; proportion of the conventional ingredients : 10 to 17% of the crude protein , 1 to 4% crude fat , crude ash 14 to 21 % , of calcium 3 to 5 % , phosphorus from 0.1 to 0.3 % . Using the Box-behnken design, silkworm excrement chlorophyll extract and sodium zinc chlorophyllin preparation process is optimized by the response surface method , solid-liquid ratio , extraction time and temperature on the yield of chlorophyll , the main consideration in the process of extraction of chlorophyll , experimental results show that : the extraction of chlorophyll optimum process as follows: 80% acetone extraction solvent , the solid-liquid ratio of 1:10, the time 4.06h , temperature 54.4 ° C , the yield in this process under the chlorophyll reached 6.05mg / g . Study on Preparation of sodium zinc chlorophyllin process , mainly investigated the saponification temperature , plus the amount of zinc and zinc -generation yield of sodium zinc chlorophyllin , experimental results show that : Ridge crest three-factor analysis greatly value the corresponding values ??are: the saponification temperature 49.3 ° C , plus the amount of zinc 2.4ml, zinc generation time of 2.0h, this condition prepared from sodium zinc chlorophyllin is expected to yield the highest , at 3.3% . The test preparation of sodium zinc chlorophyllin preliminary qualitative identification by UV-visible absorption spectroscopy and NMR , the laboratory preparation of sodium zinc chlorophyllin a product comparison , preliminary draw , these two The product does not belong to the same species .
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