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A Study on Screening of Compound Immunopotentiator Formula and Its Counteracting Effects to Immune Challenge

Author: XiaoHuaLi
Tutor: HouYongQing
School: Wuhan Polytechnic University
Course: Animal Nutrition and Feed Science
Keywords: Piglets Immunostimulants Growth performance Lymphocyte transformation rate Inflammatory cytokines Lipopolysaccharide
CLC: S853.9
Type: Master's thesis
Year: 2008
Downloads: 178
Quote: 1
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Abstract


The study group side by lymphocyte transformation test composite immunostimulants initial screening, and through feeding trials determination weaning growth performance of piglets, plasma immune indicators and antioxidant indicators to determine a composite group immunostimulants square and The application of E. coli lipopolysaccharide (LPS) stress model of the composite immunostimulants plasma hormone levels of weaned piglets inflammatory cytokine levels and plasma immune indicators. The study is divided into four test: test a composite immunostimulants Prescription vitro screening test in order to filter out the immune enhanced to better the role of Chinese herbal extract complex set of square, astragalus polysaccharide (APS), epimedium polysaccharide (EPS bidentata polysaccharide (ABPS) and Acanthopanax), saponins (ASS) and other quality than composed of four different compound preparation, for APS EPS ASS APS, APS ABPS EPS and APS ABPS ASS without fetal bovine serum RPMI-1640 culture medium formulated as 50μg/mL, 100μg/mL, 200μg/mL, 400μg/mL and 800μg/ml of five different concentrations of the solution were added to the cultured piglets peripheral blood lymphocytes, cultured for 72 hours, lymphocyte proliferation was determined by MTT reaction. The results show that: the four compound preparation in a suitable concentration could promote ConA or LPS-induced lymphocyte proliferation response, better Compound APS ASS and APS ABPS ASS. Test two composite immunostimulants on the growth performance, immune function, and the antioxidant function of the comparison of compound A (APS ASS) and Compound B (APS ABPS ASS) immunostimulants effect test chosen from the same source health of the average weight of 9.29 ± 0.53kg, age 31 ± 3d Yorkshire weaned piglets 32, were randomly divided into four dietary treatments, each treatment four replicates (columns) in each repetition (column) two piglets. Four dietary treatment groups, respectively, for the control group (basal diet without antibiotics), APS group (basal diet supplemented with 0.05% APS), Compound A group (basal diet supplemented with 0.05% Compound A immunostimulants) and Compound Group B (basal diet supplemented with 0.05% Compound B immune enhancer). Test piglets fed diets with four, 14-day feeding trial measured the growth performance of the piglets. Test period, pigs anterior vena cava blood sampling, determination of plasma the routine biochemical indicators, immune indicators and antioxidant indicators. The results show that: add the Compound A immunostimulants significant improve piglet daily gain (ADG) (P lt; 0.05), lower feed to gain ratio (F / G) (P lt; 0.05); significantly improve weaning piglets plasma γ-glutamyl transpeptidation enzymes (GGT) and inducible nitric oxide synthase (NOS) (P lt; 0.05), and plasma immunoglobulin (IgG) levels (P lt; 0.05); significantly lower plasma propionate dialdehyde (MDA) content (P lt; 0.05). Test three composite immunostimulants weaned pigs immune indicators, plasma hormone levels of inflammatory cytokines and conventional biochemical markers in order to study the compound A immunostimulants weaned piglets immune indicators, plasma hormone levels of inflammatory cytokines and routine biochemical indicators, source of test selection, the average weight of a healthy 6.51 ± 0.19kg, the average age was 23 ± 3d Duroc × Landrace × Large White weaned piglets 16, were randomly divided into two dietary treatments, each deal with four replicates (column) (column), each repeated two piglets. Two dietary treatment groups, respectively, for the control group (basal diet without antibiotics) and test group (basal diet supplemented with 0.05% Compound A immunostimulants). Period of 27d feeding trials with both diets fed piglets. Test 14d, former vena cava blood determination of plasma hormone levels of inflammatory cytokines, lymphocyte transformation rate of all test pigs per kilogram of body weight ear after intramuscular injection of bovine serum albumin (BSA) 1mg, injection After 7d blood serum BSA antibody levels. Test section 21d from the last nipple in piglets left leg medial 5cm and 5cm at subcutaneous injection of phytohemagglutinin (PHA) from the ventral midline skin allergy test. The results show that: the the weaning piglets add Compound A immunostimulants increased phytohemagglutinin (PHA) to stimulate increased skinfold thickness values ??(P lt; 0.05); reduced plasma somatostatin (SS) and glucagon hormone (PG) level as well as the activity of lactate dehydrogenase (LDH) (P lt; 0.05). The test composite immunostimulants the LPS stress weaning piglet growth performance, plasma hormones and inflammatory cytokine levels and immune index test selection sources, the health of the average weight of 6.51 ± 0.19kg, the average age was 23 ± 3d Duroc × Landrace × Large White weaned pigs to 16, were randomly divided into two dietary treatments, each treatment four replicates (bar), each repeat (column) two piglets. Two dietary treatment groups, respectively, for the control group (basal diet without antibiotics) and test group (basal diet supplemented with 0.05% Compound A immunostimulants). Piglets were fed two diets, the period 27d feeding trials. Test the first 23d the peritoneal injection 100μg/kgBW of all test pigs LPS h after injection, anterior vena cava blood collection, separation of plasma was measured plasma hormone levels and inflammatory cytokine levels and immune indexes. The results show that: the Compound A immunopotentiator significantly reduce piglet plasma leukocyte interleukin -6 (IL-6) (P lt; 0.05) and insulin (Ins) levels (P lt; 0.10), promotion of somatostatin (SS) secretion (P lt; 0.05), and increased plasma interleukin -2 (IL-2) level (P lt; 0.10) on LPS piglet performance before and after stress no significant impact. The above results show that: the Compound A immunopotentiator improve piglet growth performance, enhance immune function and the role of anti-immune stress.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Chinese veterinary science > Veterinary prescription
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