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Estrogen receptor β gene polymorphism and cerebral infarction study

Author: LiShanShan
Tutor: WangXinPing；ChenRongJie；WangShiMin
School: Tianjin Medical University
Course: Neurology
Keywords: Infarction Estrogen receptor β Gene Polymorphism
CLC: R743.3
Type: Master's thesis
Year: 2009
Downloads: 67
Quote: 0
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Abstract

Objective: This study was to investigate the Chinese Han population estrogen receptor β (estrogen receptor beta, ERβ) gene RsaI and AluI restriction enzyme polymorphism and cerebral infarction (cerebral infarction, CI) correlation. Method: 1. Data collection: cerebral infarction group 116 cases, male 63 cases, female 53 cases. Control group, 116 cases, male 53 cases, female 63 cases. Gender composition of the two groups by chi-square test, age compared by t test, in order to clear the two groups were comparable. (2) Test procedure: 2.1 each of EDTA anticoagulated blood collected study 2ml, genomic DNA isolated from the whole blood, the use of 1% agarose gel electrophoresis DNA. 2.2 the gene were designed and synthesized for the primers by polymerase chain reaction (polymerace chainreaction, PCR) amplification of the target gene in vitro, using 1% agarose gel electrophoresis of PCR product was gel imager without fragment. 2.3 The PCR-positive products were restriction fragment length polymorphism analysis (Restriction FragmentLength Polymorphism, RFLP), using 2% agarose gel electrophoresis, gel imager identified RFLP product. 2.4 pairs of PCR products were sequenced part. 3 Data Analysis: The genotype and allele frequencies calculated using gene counting method, subjects ERβ allele frequencies were compared with fourfold table χ ~ 2 test, comparing genotype frequencies associated with R × C column χ ~ 2 test. The statistical analyzes were done on SPSS 13.0 statistical package. All P values ??are two-sided test, P <0.05 was considered statistically significant. Results: 1 case and control groups for age, sex matched comparable. (2) Compared with the previous literature, ERβ gene polymorphism racial, regional differences, the East-West differences more obvious. 3.ERβ RsaI enzyme gene frequency distribution of genotypes in the case group and control group there was a significant difference (P <0.05), ie, the case group rr, RR genotype frequencies higher and the genotype Rr frequency is lower than the control group; while RsaI digestion of the allele frequencies between the two groups no significant difference (P> 0.05). 4.ERβ gene AluI digestion genotypes and allele frequency distributions between cases and control groups showed no significant difference (P> 0.05). 5.CI patients stratified according to age of onset, early-onset and late-onset patients with CI CI ERβ gene RsaI enzyme genotype frequency distributions of the difference was statistically significant (P <0.05), rr genotype in early-onset CI group The distribution frequency is higher than late-onset CI group, rr genotype may be susceptible genotype of early-onset cerebral infarction. The allele frequency distribution of RsaI digestion was no significant difference (P> 0.05). AluI digestion of each genotype, allele frequency distribution in early-onset and late-onset CI CI between patients and no significant difference. 6.CI patients stratified by sex, male and female patients with CI CI ERβ gene RsaI, AluI digestion of the genotype and allele frequency distribution showed no significant difference (P> 0.05). Conclusion ERβ gene polymorphism racial, regional differences; ERβ gene RsaI enzyme genotype may influence the susceptibility of CI and CI age of onset; while ERβ AluI restriction gene polymorphism may not affect the CI susceptibility and age at onset of CI.

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