Dissertation > Excellent graduate degree dissertation topics show

Study on the Changes of Nuclear Matrix Proteins during the Apoptosis of Esophageal Cancer EC9706 Cells by Curcumin

Author: SongJianZuo
Tutor: LiZuoFu
School: Xiamen University
Course: Cell Biology
Keywords: People EC9706 cells Nuclear matrix - intermediate filament system Nuclear matrix protein Apoptosis Curcumin
CLC: R285
Type: Master's thesis
Year: 2009
Downloads: 133
Quote: 0
Read: Download Dissertation

Abstract


Identified curcumin (curcumin, Cur) EC9706 cells apoptosis induced based on the application of selective extraction Helping transmission electron microscopy and proteomics analysis techniques, nuclear matrix - EC9706 cell apoptosis induced process intermediate filament The system configuration changes, and changes in the composition of nuclear matrix proteins to carry out systematic research. Analysis identified specific nuclear matrix protein associated with apoptosis of esophageal cancer, and to explore the role they are in the process of apoptosis in esophageal cancer, to further understanding of esophageal cancer cell apoptosis and its mechanism from a more holistic level, thus Find the new direction of the esophageal cancer cell apoptosis research. The experimental results show that after a 8μg/mL curcumin treatment, EC9706 cell proliferation was significantly inhibited cell growth inhibition rate was 69.9%; cell cycle hypodiploid (sub-G 1 of) cells peak, apoptosis rate of 23.0%, and G 2 / M phase arrest occurs; light microscopy and transmission electron microscopy results show EC9706 cells induced by curcumin treated cell shrinkage nucleocytoplasmic ratio decreases, nuclear condensation, nuclear chromatin condensation, swelling of mitochondria, endoplasmic reticulum lumen to expand, the formation of apoptotic bodies and other significant features of apoptosis; apoptosis-related proto-oncogene bcl -2 downregulation promote apoptotic gene p53, fas, bax upregulation. The selective extraction ready to light and electron microscopy showed that the curcumin treatment EC9706 cells, the nuclear matrix - intermediate filament system can maintain a more complete network structure, but the number and density of the fibers than the control group sparse and more unevenly distributed, and the presence of a local fiber breakage, nuclear matrix - intermediate filaments overall system configuration tends to disintegration; two-dimensional gel electrophoresis analysis showed 31 differentially expressed EC9706 before and after apoptosis induced by curcumin nuclear matrix proteins by mass spectrometry analysis identified 12 proteins, which in apoptosis of EC9706 cells express the upward or emerging protein 8: Ran-binding protein 3-like Mitochondrial 28S ribosomal protein S30, Rho guanine nucleotide exchange factor 19, Rap1 GTPase-activating protein 2, Hsp70-binding protein 1,2-5A-dependent ribonuclease, Adseverin, TATA elementmodulatory factor; downregulated protein 4: Probable peptidyl-tRNA hydrolase, ATP synthase mitochondrial F1 complex assembly factor 1, Serine protease hepsin, Cell division protein kinase 6. Among Adseverin outside, other proteins were first found in the nuclear matrix. The study results show that the 8μg/mL curcumin on human esophageal carcinoma EC9706 cell apoptosis has significantly induced in EC9706 cell apoptosis process, not only the nuclear matrix - intermediate filament system configuration obvious changes in the characteristics of apoptosis , and the composition of the nuclear matrix protein change significantly. Which confirmed the presence of specific nuclear matrix protein associated with esophageal cancer cell apoptosis and its role induced apoptosis of esophageal cancer.

Related Dissertations

  1. The Study on Cryopreservation and Mechanism of Freezing Injury on the Spermatozoa of Coelomactra Antiquate,S968.3
  2. Preliminary Study of Phosphorylation Effect on UGT1A3 Metabolism Activity Towards Drugs,R346
  3. The Anti-tumor Effect of CADPE and Inducing Apoptosis in Human Gastric Cancer Cells,R735.2
  4. Cloth and β- elemene combined administration of anti-tumor effect and mechanism of celecoxib,R96
  5. TRAIL in the regulation of tumor invasion CD4 ~ CD25 ~ Treg,R730.2
  6. The Study of Lead Exposure on Cyprinus Carpio Ovary Epithelial Cells,X174
  7. Effects of Diclazuril on G3PDH in Second-generation Merozoites of Eimeria Tenella,S858.31
  8. The Effects and Preliminary Study of PCV2 on Ca2+ Signal in Lymphocytes of Piglets,S858.28
  9. Effects of Angiogenic Factor on Follicular Angiogenesis and Development in Sexual Maturity Mice,S852.2
  10. Expression of β-Catenin in Pig’s Ovary and the Effect of β-Catenin on Porcine Granulosa Cells Apotosis and Steroidogenesis Related Enzyme,S828
  11. Induced Combination Apoptosis by FB1 and AFB1 in Vero Cell,S856.9
  12. The Apoptosis Mechanism Induced by Aflatoxin B1 and Deoxynivalenol in Primary Hepatocyte of Cyprinus Carpio,S856.9
  13. Effects of BMPR-IB Gene Silencing by Small Interfering RNA on Apoptosis of Porcine Pollicular Granulosa Cells and Ecpression of BMP Pathy-Way-Ralted Genes,S828
  14. The Effects of Don on Proliferation, Differentiation, and Apoptosis of Chondrocytes in Chicken,S858.31
  15. The Effect of Foxol on Apoptosis of Mouse Granule Cells,S865.13
  16. Study of Melittin on Tumor Inhibition and Part of Mechanism of Human Hepatocellular Carcinoma HepG-2 Xenograft in Nude Mice,R735.7
  17. The Protective Effect of Rat Myocardial Ischemia/reperfusion Injury Following Bone Marrow Mesenchymal Stem Cell Pretransplantation for 1 Week,R542.22
  18. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  19. The Effects of (180F-FDG on the Apoptosis of Eca-109 Esophageal Cancer Cell Line,R735.1
  20. The Signal Mechanism Underlying DON Induced Apoptosis in BHK-21 Cells,S856.9
  21. The Expression and Mechanism of NF-κ B and IL-6 after 90% Portal Vein Ligation in Rats,R657.3

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology
© 2012 www.DissertationTopic.Net  Mobile