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Effect of Chinese Herbs Bu-Shen of SOCS Genes Expression in Associated with Th2 Cytokines Counterblance in Maternal-fetal Interface

Author: ZhangZhiWei
Tutor: XuCongJian；GuiSuiZuo；WangHaiYan
School: Fudan University
Course: Obstetrics and Gynaecology
Keywords: Recurrent spontaneous abortion (RSA) SOCS Cytokines Th1/Th2 Kidney Qi medicine
CLC: R285.5
Type: Master's thesis
Year: 2009
Downloads: 57
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Abstract


Recurrent spontaneous abortion (of Recurrent spontaneous abortion, RSA) is the most common complication of pregnancy, serious harm to the reproductive health of women, its complex causes, including maternal-fetal immune tolerance the adjustment mechanism abnormal off one of the important causes. Fetal semi-allograft body of the mother, but the mother can successfully tolerated the fetus, now know that the mother will produce immune responses in non-self antigens of the fetus, this immune response to Th2, if maternal Th1-type immune response , the fetus is very easy to immune destruction exclusion, resulting in a miscarriage. This view has been widely recognized, therefore, if the deflection response of the mother to the fetus can be made to shift from Th1 to Th2 immune advantage, it is possible to successful pregnancy. Rat model of bromocriptine induced abortion, the abortion rate of 75 to 80%, with a low of PRL, P levels of endocrine function and pathophysiological features of bias Th1-type immune response, in line with clinical low of PRL, P levels of endocrine function and immune recognition dysfunction RSA characteristics. According to Chinese medical theory, the Kidneys Reproductive cellular network system in the kidney \Without a solid, established the kidney Qi Fang, the treatment of spontaneous abortion miscarriage efficacy, many studies of kidney essence in medicine kidney function and immune function is closely related to past many clinical studies and animal experiments also support this argument and found that the kidney Qi Fang miscarriage successfully rats decidual expression of Th2 immune advantage. Recently discovered cytokine signaling negative regulator of (a Suppressors CytokineSignaling, SOCSs) There are eight clear function of SOCS1, SOCS2, SOCS3. SOCS family involved in the proliferation and differentiation of Th cells through a variety of signal transduction pathways involved in the regulation of cytokine balance. Significance in regulating the body's complex physiological immune endocrine function. Speculated expression in maternal-fetal immune interface SOCS, and participate in the regulation of cytokine balance, maintenance of pregnancy Th2 type immune advantage further explore traditional Chinese medicine treatment of this model decidual SOCS expression and maternal-fetal immune advantage induced direction. Objective: To investigate the the kidney Qi Fang regulation of SOCS genes on the maternal-fetal interface Th2 cytokine differentiation inducing effect: an animal model and treatment: 69 10 to 12 weeks of age, not pregnant female SD rats, weighing 250 to 300 g, found proestrus the night in the same cage, rats every other day morning see the vaginal plug smears see sperm for the first day of pregnancy, in pregnant sixth day of the establishment of the bromocriptine induced abortion model 2 experimental groups: randomly divided into six groups: A: model Chinese medicine treatment: model of progesterone treatment, C: prolactin treatment D: model group, E: normal pregnant rats, F: nonpregnant rats. experimental methods: in pregnant observed 12 days pregnancy outcome, take slough film, endometrial immunohistochemical techniques to SOCS1, SOCS2, SOCS3 positioning and analysis with relative quantification Real time PCR method to detect the rats decidual endometrial SOCS1 was SOCS2, SOCS3 gene expression, ELISA method detecting cytokines (TNF-a, IFN-γ, IL-4, IL-10) in each group in the model expression. Statistical methods: SPSS15.0 statistical analysis of the median and percentile and KW test speramen correlation analysis with P <0.05 was considered statistically significant. Results: 1. Pregnancy outcome comparison: TCM treatment group, progesterone treatment group, prolactin treatment group, the pregnancy rate of the normal group of pregnant rats, the total number of fetuses, the average litter size of the average weight of the uterus was significantly higher than bromocriptine abortion model group (P <0.01). 2. Groups of SOCS1, SOCS2, SOCS3 protein expression: immunohistochemistry results show that groups the SOCS mainly expressed in decidual stromal glands of SOCS1, SOCS2, SOCS3 in Bromocriptine model group was significantly lower than miscarriage treatment groups (P <0.01). 3 groups of SOCS1, SOCS2, SOCS3 mRNA relative expression: Real time PCR results show that SOCS1, SOCS2, SOCS3 expression prompted miscarriage treatment group was significantly higher than the abortion model group, which SOCS1, SOCS2 expression of progesterone miscarriage, lactation Su miscarriage highest treatment group (P <0.05); SOCS3 expression to Chinese medicine miscarriage highest treatment group (P <0.05). The 4.ELISA detect cytokine levels: the the abortion model groups IL-10/TNF-a ratio decreased (P <0.001) of Th1/Th2 bias Th1-type away from the Th2-type cause miscarriage occurred; miscarriage and normal pregnancy Th2-type Cytokines dominant. The correlation analysis of SOCS1, SOCS2, of SOCS3 IL-10/TNF-a ratio showed a positive correlation (P <0.01) Conclusion: 1. Bromocriptine induced abortion rat model of SOCS1, SOCS2, SOCS3 expression is relatively lower than miscarriage treatment groups the SOCS high expression maternofetal of immune induction of Th2 immune advantage to form a link. 2. The kidney Qi Chinese medicine treatment of abortion allows SOCS expression increased, adjust the maternal-fetal interface, enhanced immune tolerance played maintain early pregnancy role.

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