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Mechanism Study on Effects of Celery to Reproductive System in Male Mice

Author: LiHui
Tutor: LiZhiLan
School: Lanzhou University
Course: Occupational and Environmental Health
Keywords: Celery Male mice Reproductive system Serum biochemical Antioxidant Ultrastructure Cell cycle
CLC: R151
Type: Master's thesis
Year: 2009
Downloads: 67
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Abstract


Objective To explore the effect and mechanism of the celery on the male reproductive system, and to provide a basis for the development and utilization of celery. Method the SPF grade Kunming 240 healthy adult male mice were randomly divided into two time groups, namely 7d, 56d group. Each time group animals according to body weight and randomly divided into 4 groups: negative control group of 30, 30 low-dose group, middle dose group 30, the high-dose group, 30. 0.3ml saline orally every morning at 8:30 to the negative control group, the remaining dose groups orally 0.3ml different concentrations of celery juice, two-time group were gavage end recovery 7d 14d randomly selected 10 The mice were sacrificed, drawing. (1) separation of the liver, spleen, kidney, testis and epididymis were weighed and calculated organ coefficient. (2) Determination of hemoglobin, serum alanine aminotransferase enzyme (ALT), aspartate aminotransferase the enzymatic (AST), the ALT / the AST, albumin (ALB), globulin (GLO), A / G, alkali phosphatase (ALP) content; detection of serum testosterone (T), luteinizing hormone (LH) and follicle-stimulating hormone (FSH) levels. (3) Determination of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), and total antioxidant capacity (T-AOC) activity. (4) light microscope to observe the morphological changes of the testicular tissue and testicular ultrastructure changes were observed under transmission electron microscopy. (5) detection of somatic cell cycle of mouse spermatogenic cells for various times. Results (1) gavage 56d after liver index, middle dose group with the control group, the high-dose group compared with the low-dose group differences were statistically significant (P <0.05), recovery 7d, 14d organ coefficient of each dose group the difference was not statistically significant; 7d organ coefficient of each dose group differences were not statistically significance (P> 0.05). (2) the hemoglobin content of each dose group showed no significant difference (P> 0.05); gavage the 7d of high dose group T content with the control group difference was statistically significant (P = 0.045), recovery 7d, 14d after each dose group, the difference was not statistically significant (P> 0.05), the 56d group each dose group FSH, LH, T the differences were not statistically significant (P> 0.05); gavage 56d ALT, AST high dose group other dose group, the the ALP low dose group and the control group, the the ALB high-dose group and the control group and low dose group, A / G middle dose group and the control group were statistically significant (P <0.05) after recovery 7d in ALB of the high-dose group and low dose group differences were statistically significant, the difference was statistically significant (P <0.05) of the high dose group the GLO with low dose group, there was no statistically significant difference in the biochemical markers of recovery 14d each dose group ( P> 0.05). (3) 7d group each dose group SOD, GSH-Px, MDA, T-AOC activity showed no significant difference (P> 0.05), 56d group was given the activities of SOD, GSH-Px, of MDA, T-AOC activity in each dose group, the difference was not statistically significant (P> 0.05); recovery 7d after high doses of GSH-Px activity and the control group, low, middle dose group differences were statistically significant, high-dose group, the activity of T-AOC difference was statistically significant (P <0.05) compared with the middle dose group; recovery 14d high doses of GSH-Px activity compared with control group was statistically significant, high doses of T-AOC activity with the control group, the low-dose group difference are was statistically significant (P <0.05). (4) Light microscopy no significant pathological changes in the mouse testis of each experimental group; the electron microscope mice testicular tissue ultrastructure have some changes, mainly for stromal cell particles increased mitochondrial expansion, support cell edema, endoplasmic reticulum, mitochondria expansion, the formation of vacuoles in the sperm cells, 7d recovery 7d damage some heavier recovery 7d 56d group ultrastructural damage relief. (5) FCM measurement results show that the 56d group was given high-dose group mouse testis the 2C cell count and middle dose group difference was statistically significant (P <0.05), the 4C cell number compared with control group differences were statistically significance (P <0.05), mouse testis, and the number of S phase of the seminiferous epithelium cells with low and medium dose difference was statistically significant (P <0.05), G2 / M spermatogenic number of epithelial cells in the control group, low and medium dose the difference was statistically significant (P <0.05). High-dose group 1C: 2C compared with the middle dose group difference was statistically significant 4C: 2C with the control group, middle dose group difference was statistically significant. Recovery 7d, 14d after each dose group showed no significant difference (P> 0.05). Conclusions (1) celery have a certain impact on the structure and function of the liver of mice, but return to normal. (2) celery mice Hb levels had no significant effect, serum T can cause reduced secretion of content and serum biochemical parameters change. (3) celery can increase the total antioxidant capacity of the mouse testis. (4) celery mouse testis ultrastructure has a certain impact on recovery 14d degree of damage has been reduced. (5) celery male mice spermatogenic inhibition.

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CLC: > Medicine, health > Preventive Medicine,Health > Nutrition, hygiene,food hygiene > Nutrition
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