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Preparation and Characterization of P(DEA-co-AA) Microgels and Its Role in Horseradish Peroxidase Immobilization

Author: ZhangYaPing
Tutor: FangJian
School: Lanzhou University
Course: Physical and chemical
Keywords: N, N- diethyl acrylamide (DEA) P (DEA-co-AA) Thermosensitive Microgel VPTT Macromolecule Enzyme immobilization Horseradish peroxidase (HRP) Phenolic Wastewater Reaction kinetics
CLC: O631.3
Type: Master's thesis
Year: 2009
Downloads: 105
Quote: 0
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Abstract


Article main monomer, N, N-diethyl acrylamide (DEA) modified by copolymerization, the introduction of the hydrophilic monomer is acrylic acid (AA), with the emulsion polymerization method to prepare a small particle size, good monodisperse, new having a temperature response performance of poly (N, N-diethyl-acrylamide-co-acrylic acid) (P (DEA-co-AA)) microgel, systematic study of the resulting microgel morphology, monodisperse and the phase transition behavior; microgel fixed horseradish peroxidase enzyme (HRP) used for enzyme immobilization carrier, study enzyme immobilization conditions and the structural properties of the immobilized enzyme, and as research object Phenolic Wastewater explore it immobilized enzyme catalytic oxidation effect and catalytic kinetics. The main content includes the following three parts: 1. N, N-diethylacrylamide amide (DEA)-based monomer, acrylic acid (AA) as a comonomer, the soap-free emulsion polymerization method, the synthesis of a series of temperature-sensitive resistance microgel P (DEA-co-AA). Infrared spectroscopy (IR), scanning electron microscopy (SEM), characterized by turbidity method and dynamic light scattering (DLS). The AA content of microgels morphology, Volume the phase transition temperature (VPTT) and swell ratio. The results show that: the synthesis of P (DEA-co-AA) microgels monodispersed spherical particles, the particle size of the dry gel increases with the increase of acrylic acid in the mole fraction of the microgel. pH 7.0, the volume of the microgel with increasing content of AA phase transition temperature (VPTT) gradually increased; AA content of VPTT pH 4.0. Increasing the the microgel swelling ratio increased as the AA content. The HRP enzyme is fixed on the P (DEA-co-AA) microgel, IR spectra showed the presence of hydrogen bonding interactions between the enzyme and the microgel, the HRP enzyme not only by physical adsorption and parcels, but also fixed by hydrogen bonds in microgels carrier. Fixed, the pH of the system have a greater impact on the activity and the immobilization rate: pH value is lower, the immobilization rate is higher, the acidic environment of the microgel fixed of AA unit itself ionization produced can achieve The ideal immobilization effect, adding a buffer solution, a small electrolyte introduced, it will affect the immobilization of enzymes. Immobilized enzyme that is basically completed within 60 min; the comprehensive enzyme immobilization rate and immobilized enzyme catalytic performance and from an economic standpoint drawn, vector dosage should take approximately 0.82 mg / u microgels. Good enzyme immobilization experimental reproducibility. Under suitable conditions, remove phenol from wastewater using immobilized HRP-catalyzed oxidation of phenol removal rate of more than 96%. Immobilized enzyme solution enzymatic kinetics studies have shown that the solution better enzyme affinity for phenol in phenol oxidation reaction, but the immobilized enzyme showed greater maximum reaction rate. P (DEA-co-AA) the performance of the microgels fixed HRP study also shows, immobilized in the micro gel VPTT vertically HRP could be reversibly turned on or off the activity of the enzyme. In the same time, the immobilized enzyme reused 5 times, phenol removal remains at more than 50%. The immobilized enzyme in a desiccator for 2 months at room temperature, about 94% of phenol removal remains. The immobilized enzyme has very good thermal stability, storage stability and reusability, microgels P (DEA-co-AA) is a useful potential applications of the enzyme fixed carrier.

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CLC: > Mathematical sciences and chemical > Chemistry > Polymer chemistry ( polymer ) > Polymer physics and physical chemistry of polymers > The chemical nature of polymers
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