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This study established the carp plasma and muscle of mebendazole content in HPLC detection method, and mebendazole the carp plasma pharmacokinetics and elimination of residual muscle were studied. This test first establish HPLC method for the the mebendazole content of the carp plasma and muscle. Plasma samples using ethyl acetate as the extraction agent, acetonitrile: KH 2 PO 4 solution (0.05 mol / L, pH2.5) (40:60, V / V ) as the mobile phase for HPLC analysis. The muscle sample first with ethyl acetate to the protein to the fat, and then with n-hexane, and finally with acetonitrile: the KH 2 PO 4 solution (0.05mol / L) (35:65 , V / V) as the mobile phase for HPLC analysis. Mebendazole in plasma recovery was 86.45% to 90.71%, the coefficient of variation ranging from 5.78% to 8.57%, the LOD and LOQ 0.01mg / L and 0.05 mg / L, respectively; of mebendazole muscle recovery of 87.54 % to 93.48%, the coefficient of variation ranging from of 4.88% ~~ 5.97% LOD and LOQ for 0.005mg/kg 0.01mg/kg. A total of 95 healthy carp, including 55 for the pharmacokinetic study, 40 for residual elimination of research. Imidazole oral liquid medicine to 5mg/mL toluene pharmacokinetic group carp a single oral dose, the dose of 10mg/kg bw, respectively administered 6,12,24,36,48,60,72,84,96,144 h , carp tail vein blood samples were collected. Mebendazole oral liquid medicine the residue analysis group given orally 5mg/mL the, a dose of 10mg/kg bw, once a day for five consecutive days, respectively, in 1,2,4,6,10,16,24 d after the administration collected carp muscle samples. Toluene in the concentration of imidazole in the plasma and muscle were determined by reverse phase HPLC chromatography. 3p97 pharmacokinetic program software processing drugs - when data. Pharmacokinetic study, after the carp a single oral dose mebendazole plasma concentration - time data are consistent with a one-compartment open model, its main pharmacokinetic parameters were: t 1/2 (ka) 16.202 ± 1.256h, t 1/2 (ke) 18.582 ± 2.551h, T (peak) 32.058 ± 0.883h, K a 0.043 ± 0.003h -1 , K e 0.038 ± 0.005h -1 , C (max) 0.336 ± 0.012mg / L, AUC 22.902 ± 2.270mg / L · h. The results show that, in water temperatures of 18 ± 2 ℃, mebendazole slowly absorbed in crucian carp, small volume of distribution and longer half-life and residence time, lower blood concentration. Residual eliminate water temperatures of 18 ± 2 ℃, carp multi-dose oral mebendazole, the 4d muscle after stopping mebendazole concentration reaches 0.696mg/kg test, fell to 16d 0.042mg/kg, 24d muscle in mebendazole content below the limit of detection (0.005mg/kg), so in this temperature range, the carp multi-dose oral mebendazole after a withdrawal period of at least 24d.
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