|
Eels known as eels, is one of the valuable economic fish the eel aquaculture production in China ranks first in the world, the main species farmed European eel (Anguilla anguilla (Linnaeus)) and the Japanese eel (Anguilla japonica (Temminck et Schlegel)), eels ( Anguilla rostrata (Lesueur)) is also in some regions began attempts to raise. In the breeding process, various diseases continue to occur, bacterial disease caused harm. However, the rapid detection of live eel pathogen kit small, yet effective method. In this study, the use of freshly prepared 10 pathogen immune serum and laboratory previously developed 23 kinds serum composed of rapid diagnostic kits, and with this kit eel main support areas of Fujian Province and Guangdong Province in different regions, different time, different specifications of eel are suffering from pathogens site rapid testing, the results are as follows: 1. applications in our laboratory isolated 10 pathogens vaccination New Zealand white rabbits were obtained serum titer of 1024 and above 8; serotypes efficiency $ 512; titer 256.2 10 29 pathogens (including preparation of immune serum 10 pathogens) slide agglutination cross-reactive immune serum separation of the lab results showed: no cross reaction of sera 7 species, there are cross-reactive sera 3 species, the number of cross strains 1, 2 and 4, respectively, the cross strains agglutination titer antiserum strain agglutination titer ratios up to 1/2, a minimum of 1 / 8.3. HiTrap Protein A HP affinity column purified AKTApurifier-100 protein purification instrument cross-reaction with 3 kinds of serum, after purification, the purified serum protein content ratios calculated recovery rate, while the purification factor is calculated using the ratio of specific activity before and after the purification, results show that: the sample purification, the maximum recovery of 30%, the lowest rate of 7.5%, the highest purification factor was 85, a minimum purification factor 3.1. And purified the three kinds of serum with 29 pathogens slide agglutination test No significant cross reaction. 4. Use of available rapid diagnostic kits to detect 33 kinds of eel pathogens in four regions of Fujian Province (Mindong, Taiwanese, western Fujian, northern Fujian) Guangdong Province, Taishan City, different time, different regions, different types of eel pathogens site rapid testing, results show: the European eel, Fujian Province, over these two time periods of 4 to 6 and 8 to October 12 pathogens detected, of which 4 to 6 months, 9, 8 to 10 months detected 10 strains, six pathogens can not exist simultaneously in two time periods, different time eel pathogen infection. European eel in April to June, were detected in the three regions detected 9 of pathogens, only one pathogen can occur simultaneously in three regions; were detected in 8 to 10 months, the four regions, detected 9 pathogens, two pathogens simultaneously in four regions; instructions at the same time in different parts of the pathogen difference. 8 to 10 months of Taishan City, Guangdong Province, Japanese eel detection detected 5 of pathogens, including three Japanese eel farms in Fujian Province was also detected, indicating that both Japanese eel pathogen infection did not differ significantly. Both European eel were detected in the 12 pathogens, Japanese eel detected a total of 13, only three exist in two eel, description of different types of eel pathogens varied.
|