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Research on Pollen Storage and Controlled Pollination Techniques of Eucalyptus

Author: WangWei
Tutor: XuJianMin；LuZuoHua
School: Chinese Academy of Forestry
Course: Tree Genetics and Breeding
Keywords: Eucalyptus Pollen Storage Pollen viability Grafting Controlled pollination
CLC: S792.39
Type: Master's thesis
Year: 2009
Downloads: 124
Quote: 1
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Abstract


Eucalyptus (Eucalyptus) crossbreeding eucalyptus genetic improvement. Heterosis outstanding achievements in modern breeding, asexual reproduction sexual seed use can greatly improve the production of eucalyptus plantations. Eucalyptus hybrid breeding in China began in the 1960s, dating back more than 50 years of history, cross-breeding techniques have also experienced a series of reforms, and systematic technology system. In this paper, on the basis of previous studies, phenological observations from the eucalyptus dwarf breeding nursery establishment, pollen storage and artificial control pollination techniques to conduct a more in-depth investigation, exploration and analysis of research to come to the the following conclusions: (1) through two years of Eucalyptus flowering material climate of observation and combination of experts over the years observed summarize, basically mastered the South China eucalyptus case of main afforestation tree species of flowering climate, timely conduct Eucalyptus genus interspecific and intraspecific hybridization provided favorable conditions. (2) dwarf breeding technology research, summed up a set of practical eucalyptus grafting technology, application of plant growth retardants paclobutrazol on eucalyptus dwarf promoting flower studies. The results show that the: eucalyptus-grafting suitable temperature for 18 ~ 28 ℃, and the a suitable grafting method for docking Law; MET dwarfing effect of Eucalyptus tree was dwarfed treated section shortened, tillering increase, but the the reaction of the different clones of paclobutrazol differences. (3) at room temperature storage, low temperature storage (3-5 ℃), cryogenic storage (-15 ~ -20 ℃), and ultra-low temperature frozen storage (lt; -70 ℃) eucalyptus pollen germination in vitro determination tests, results : pollen medium the most suitable for Eucalyptus pollen germination 20% sucrose 6% agar 100ppm boric acid; storage methods and storage time significantly affect pollen viability; stored at room temperature vacuum urophylla and Eucalyptus grandis pollen storage 30d after germination rate decreased to less than 10% of Eucalyptus urophylla × Eucalyptus grandis and Dunn Eucalyptus pollen storage 50d pollen germination rate reduced to below 10%, freezing low temperature and ultra low temperature storage year pollen germination rate remained at 35% ; different thawing on pollen germination rate significantly influence best in 38 ° C water bath for 3min thaw effect. (4) to carry out a one-time control pollination (OSP) and artificially induced pistil first cooked (AIP) control the comparative test control pollination pollination treatment with the traditional three-step process (TCP), the results showed that: OSP pollination 1 (interception stigma 1 / 3 length) here fruit rate and the effective number of seeds obtained were significantly higher than that of the traditional control pollination; the the AIP pollination processing yellow fruit and a light white fruit get a more traditional control pollination higher rate of fruit processing, fruit rates were 55.55 percent and 41.72 percent higher.

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CLC: > Agricultural Sciences > Forestry > Forest tree species > Broad-leaved trees > Eucalyptus
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