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Good genetic transformation receptor system was created for genetic transformation of the premise and foundation. Lily established through tissue culture regeneration system for the genetic transformation of providing a suitable acceptor material, the current success of the lily has more than 40 kinds of tissue culture methods. Somatic embryogenesis regeneration system has a reproduction rate, conversion rate is small, less chimera, somaclonal variation, etc, and is ideal for conversion receptor system. At present, in Aralia lily, Lilium oriental lily varieties and varieties induced somatic embryos. However, somatic embryogenesis required conditions are high, low incidence. In view of this, the experimental study of the Chinese origin of Lanzhou lily (Lilium davidii var.unicolor), Tong Jiang lily (Lilium sargentiae), as well as their predecessors had not studied the current popular on the market Lilium 'Whitehaven' (' White Heaven '),' Yellow Storm '(' Yelloween ') and other varieties of tissue culture systems and induction of somatic embryogenesis system, the conditions of somatic embryogenesis and media types are discussed, resolved lily somatic embryo preservation process browning problems, main results are as follows: (1) establishment of Lily and 'Whitehaven' (Lilium hybrid cultivars), 'Yellow Storm' (OT class lily varieties) in vitro propagation system. Lanzhou lily optimum differentiation medium was MS 6-BA1.0 mg / L NAA0.1 mg / L, the differentiation rate of 100%; optimal proliferation medium was MS 6-BA1.0 mg / L NAA0.5mg / L, bud multiplication 4.04; optimum rooting medium was 1/2MS NAA0.8 mg / L, the average root number 12.22. 'Whitehaven' scales optimal differentiation medium was MS 6-BA1.5 mg/LNAA0.1mg/L, differentiation rate of 100%; optimal proliferation medium was MS 6-BA1.0 mg / L NAA0.45 mg / L, bud multiplication 4.02; optimum rooting medium was 1/2MS NAA0.8 mg / L, the average root number 10.87. 'Yellow Storm' scales optimum differentiation medium was MS 6-BA0.5 mg / L NAA0.1 mg / L 2,4-D0 .1 mg / L, the differentiation rate 97.44%; optimal proliferation medium was MS 6 -BA1.0 mg / L NAA0.1mg / L, bud multiplication 4.02; optimum rooting medium was 1/2MS NAA0.8 mg / L, the average root number 8.27. Choose from three lily bulblets whole differentiation rate, appropriate to add a low concentration of 2,4-D in favor lily buds, high concentrations of 2,4-D is conducive to the formation of callus. (2) filter out Lily, 'Whitehaven', 'yellow storm' optimum medium induced bulblets. Among them, the sugar concentration in Lanzhou lily 80g / L medium BULB rate of 100%, the small bulb diameter and weight gain has reached the maximum, respectively, were 1.243cm and 0.460g; 'Whitehaven' 80g sugar concentration in / L, 'yellow storm' in glucose concentration 100g / L medium BULB rate of 100%. 'Whitehaven' is sucrose 60g / L medium bulblets maximum diameter and weight, respectively, 1.269cm and 0.562g; 'yellow storm' in sucrose 80g / L, the diameter and weight of bulblets maximum, respectively 1.289cm and 0.782g. (3) use the 'Whitehaven' scales for somatic embryo induction culture conditions found in dark outer scales embryo induction rate and the average maximum number of somatic embryos. Through three factors and three levels orthogonal test, screening out 'Whitehaven' somatic embryogenesis best medium for MS 6-BA (0 ~ 0.2) mg / L 2,4-D0 .5 mg / L LH 400 mg / L, induction rate of 33.33%. Studies show, LH on the induction of somatic embryos play a leading role. Embryos saved the best medium for MS 6-BA0.5 mg / L 2,4-D0 .2 mg / L LH 1000 mg / L. In 0.5 mg/L6-BA in MS medium supplemented with 4.5g / L AC, is not only the largest amount of embryo growth, growth is good, and the longest period of subculture, not browning. (4) the use of Lily, 'Whitehaven' scales, Lily, Wodan, 'yellow storm' oriental lily hybrid system four varieties of floral organs and Lily, 'Whitehaven' aseptic blades embryogenic callus Tissue Culture, screening out the most easy to produce embryogenic callus genotype and explants, and the best embryogenic callus induction medium. Lily of the best induction medium MS 6-BA0.5 mg / L 2,4-D0 .2 mg / L; 'Whitehaven' best induction medium MS 6-BA0.5mg / L 2, 4-D0.5 mg / L. Add 2,4-D more useful than adding KT Lily, 'Whitehaven' embryogenic callus induction. Deep red petals and filaments in MS 6-BA0.5 mg / L 2,4-D1 .0 mg / L medium induced callus; 'Yellow Storm' filigree best place embryogenic callus medium was MS 6-BA0.2 mg / L 2,4-D0 .5 mg / L TDZ1.0 mg / L, pedicels embryogenic callus occurred optimum medium for MS 6-BA 0.2 mg / L 2 ,4-D1 .0 mg / L TDZ 0.1 mg / L. Oriental lilies callus induction rate of organ differ because of the variety. Lily and 'Whitehaven' plantlets blades respectively MS NAA1.0 mg / L TDZ0.3mg / L and MS NAA1.0 mg / L TDZ0.mg/L1 small medium observed occurrence of callus . (5) Through the 'Whitehaven' bulbs produce callus and 'yellow storm' stalk resulting indirect callus induction, somatic embryos screened optimum medium occurs indirectly, 'Whitehaven' callus proliferation and induction of somatic embryogenesis optimum medium for MS 6-BA0.5 mg / L LH800 mg / L, while the 'yellow storm' peduncle callus proliferation and induction of somatic embryogenesis, the optimal group is MS 6-BA0.5 mg / L LH600 mg / L. In this study, hormone type, concentration, and a variety of environmental conditions on plantlets growth and the impact of somatic embryogenesis screened induce somatic embryos lily explants and suitable medium for transgenic breeding lilies provide a good intermediate receptors. The current study is still in the stage of tissue culture, the next step may be established by the somatic embryo genetic transformation system of lily, lily and induction of somatic embryos cellular and molecular level in-depth study.
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