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Forestry insect virus as an efficient and harmless loyal and strong pesticides now in forest protection has played a significant role. But not wide enough in the utilization of forestry, mainly because of the viral pest control than chemical pesticides long incubation period, there is no the latter then immediate effect, users have questioned virus pest control. The effect on the prevention and control of insect viruses merely morphological observation stage of the virus, the purpose of this experiment is to establish several insect virus nucleic acid-based detection techniques, which blank fill insects virus detection in molecular biology, at the same time application of these techniques detected for vertical transmission and prevention of insect viruses to monitor the effect of low-level presence of the virus in the insects, microscopic, molecular biology conclusive evidence, further argues Applied Entomology the Virus Prevention forestry pests feasibility, to ensure that forest ecosystems to maintain stable. Designed two pairs of primers under HcNPV pe38 gene using the PCR assay HcNPV genomic DNA were amplified length of 994 and 614 bp fragments for HcNPV pe38 gene fragment was determined by sequencing. Two pairs of primers were used to detect total DNA and Hyphantria worm polyhedrosis virus obtained, which detects the minimum amount of 1 fg worm total DNA and 3 to 4 OBs / mL. With different concentrations of virus infection test insects after different time whichever hemolymph detected by PCR method. The results show that the amount of inoculation of 3.53 × 109 OBs / mL, 36 h after inoculation, the amount of virus can be detected in DNA; 3 ~ 4 OBs / mL, 120 h can be detected. The virus can be detected in time with the then decreasing drug concentration and delayed. From Qinhuangdao application of woodland and HcNPV the application of viral pest woodland collection fall webworm wild larvae, whichever is the hemolymph to do template for PCR amplification, the result is obvious instructions before the establishment of the method can be applied to the practice, while to illustrate HcNPV the fall webworm populations in the region has continued to control role. Two pairs of primers were designed according to ClanGV egt gene, take advantage of the PCR method ClanGV genomic DNA, amplified fragments of 401 and 491bp respectively, identified as ClanGV egt gene fragments were sequenced. The application of two pairs the primers detected ClanGV virus suspension, also successfully amplified target gene fragment, two higher specificity, you can use it to detect the presence of the ClanGV, which lay the foundation for the field detection of insect viruses.
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