|
The clinical application of the research background Oral Implantology dentistry in the history of the 20th century most people will urge the purpose of a big progress in clinical practice, a lot of dental implants patient line are subject to a variety of reasons Bone Augmentation surgery, but the bone incremental success largely depends on the performance of guided bone regeneration of bone substitute materials. Through continuous clinical treatment, people fully understand the important value for guided bone regeneration and bone substitutes, bone substitutes through scientific research and efforts to improve bone guide and the the osteoinductive ability to promote the role of bone formation. From the structure and physical properties of bone substitutes, calcium and phosphorus composition is more reasonable, biocompatible better three-dimensional structure is more close to human cancellous bone, bone substitutes for bone guiding role will be able to play the greatest degree, and the structure and ingredients also affect the ability of bone substitutes can absorb reconstruction. Commonly used bone substitutes can only provide guidance on physical scaffold for new bone growth, and then to induce cellular components and thus promote bone metabolism still need to add a stimulating factor, by affecting the differentiation of osteoblasts and osteoclasts, proliferation, maturation and activation, role in bone reconstruction process. Review current commonly used bone substitute, they have good biocompatibility, but there are many defects, such as autologous bone easily absorbed unable to maintain adequate bone mass the basic inorganic bone decalcification Mavericks can not absorb impact plant the bone area bone remodeling some of the features are largely influence the effect of guided bone regeneration. In addition the study found bone metabolism stimulating factor also exists a preparation difficult, high cost and unpredictable side effects of the difficulty in the practical application, the future is still need for further research to be improved rear can be really used in clinical. People are many studies trying to find the osteoblasts have higher performance alternative materials, as well as more practical value bone-stimulating factor, and recently, the research and development of new coral hydroxyapatite artificial bone, and fluoride ions on implant The discovery of bone interface osteogenic effect, will bring more high-performance manufacturing composite bone substitute material and its clinical application become possible. This thesis through in vivo assessment of the performance of new coral hydroxyapatite artificial bone on guided bone regeneration capacity, as well as observe this based on the concentration of the fluoride ion bone substitutes guided bone regeneration performance changes brought about. Purpose to explore Y500R coral bone hydroxyapatite (CHA) bone in the rabbit craniofacial osteogenic effect of fluoride ions on Y500R coral hydroxyapatite (CHA) bone to bone effect. Method 1. Diameter 11.5mm Medical titanium rod, machining titanium isolation into certain specifications Cup, fully polished multiple ultrasonic surface cleaning and chemical reagents, packaging, autoclavable, dry spare. Y500R CHA artificial bone was prepared Unit particle size Φ0.25-1.0mm, to configure a certain concentration of fluoride solution, CHA bone soaked in NaF solution, so that fluoride ions adsorbed on the surface of the bone meal in an oven ( 80 ° C) drying to fully dry state, respectively, packaging, sterilization (cobalt 60) spare. 48 New Zealand white rabbits were randomly divided into A, B, C three groups, A group of 24 rabbits were trapped the fossa and fresh bone wound through surgical methods in the the craniofacial Preparation ring bone, fixed a equipped with autologous bone titanium isolation cap and a full titanium isolation cap of bone marrow blood group B, 12 rabbits were each fixed craniofacial two equipped with the titanium Y500R CHA artificial isolation cap, C group of 12 white rabbits, craniofacial fixed two titanium containing fluorinated CHA artificial isolation cap, loosen skin tight suture to close the wound, three days after intramuscular injection of penicillin 200,000 units to prevent infection, separate feeding. After the 10th, the 11th, the subcutaneous injection of tetracycline, before being killed on the 3rd, 4th subcutaneous injection of calcium calcein. 2,4,6 weeks after respectively throughout the group A 8 killed animals to kill four animals, while the group B, C throughout the titanium cap is removed and trimmed together with the skull complete after 10% Formalin 4 ° C. soaked fixed, observation of specimens, dehydrated in alcohol, chloroform soaked transparent, production of plastic-embedded with metal hard tissue slices under a fluorescence microscope after toluidine blue - acid fuchsin staining perishable tissue optical microscope Observation, analysis by applying image measurement software on the hard tissue slices and obtain relevant experimental data, and the experimental data were statistically compared between the different groups analysis, draw the corresponding conclusions of the experiment. Results 1. Growth of experimental animals after surgery recovered well wound inflammation without dehiscence titanium the isolation cap securely without loose animals eating activities Accessibility. Cut white rabbits head skin 2. Specimens observed visible the fibers soft tissue titanium isolation cap tightly wrapped, no cavity is formed, titanium the isolation cap with bone surface chimeric good, secure loose in the effective shielding of the connective tissue long into, and the formation of new bone growth ideal space. Titanium isolation cap wrapped around the growth of the soft tissue is removed, the slow rotation and removal of the titanium cap, remove titanium cup visible structured consistent cylindrical shape of the bone substitute, no fibrous tissue ingrowth of new bone from the bone facing Growth attached calcein orange red clear discrimination for comparison analysis. Fluorinated the CHA group nascent bone than the CHA group more osteogenic effect is more pronounced. 3 new bone attached fluorescent dyes emit green and yellow fluorescence was observed under a fluorescence microscope, fluorescent control the height and area of ??new bone formation between samples clear, comparable. The area of ??the fluorescence emitted by the measurement of new bone in titanium cup: 2 weeks and 4 Zhou Xinsheng bone mass fluorinated CHA group> CHA group> autograft group> blank group, six weeks specimens of the amount of new bone fluorinated CHA group> CHA group ≈ autograft group> blank group. Toluidine blue. Observed under an optical microscope - acid fuchsin staining visible new bone from the bone ingrowth titanium isolation cap-oriented, mature bone is dyed crimson, osteoid is stained blue. 2 weeks less the amount of new bone blank group, almost no new bone formation, while the other three groups are visible upward growth of bone from the bone wound, fluoride CHA group up to the amount of new bone, followed by the CHA group autograft group than fill Such is Life substitutes group of new bone was significantly less; 4 weeks after each group are visible more trabecular bone formation, fluorinated CHA composition of bone effects still significantly than any other group, while the control group also visible certain amount of new bone ingrowth; six weeks after the new bone has matured, dense plate shape, the osteoid fully occupy titanium isolation cap space, fluorine-containing the CHA composition bone effects of the most significant. 5. Statistical analysis of the results: After two weeks, since the new bone autograft group, blank group, fluorinated CHA, CHA group fluorescence area are: (2.86 ± 0.43) the mm ~~ 2 (1.09 ± 0.2) mm to 2 After 4 weeks, (7.38 ± 0.57) mm ~ 2, (4.46 ± 0.38) mm ~ 2, analysis of variance results: fluorinated CHA group> CHA group> from the autograft group> blank group (P <0.01); self-body bone group blank group, group of fluorinated CHA, CHA group of new bone fluorescence area are: (6.67 ± 0.74) mm ~ 2, (3.68 ± 0.72) mm ~ 2, (11.78 ± 0.78) mm ~ 2, (9.22 ± 0.52 ) mm ~ 2, analysis of variance results: Fluorinated CHA group> CHA group> autograft group> blank group (P <0.01); six weeks after autograft group, blank group, fluorinated CHA group, the CHA group's new bone fluorescent area are: (10.24 ± 0.54) mm ~~ 2 (5.5 ± 0.43) mm ~~ 2 (13.91 ± 0.60) mm ~~ 2 (10.47 ± 0.40) mm, analysis of variance results: fluorine-containing CHA group> The CHA group ≈ autograft group (group P> 0.05)> control group (P <0.01). Conclusion. Titanium isolation cap production of guided bone regeneration animal models operating method is simple, minimally invasive, and the reliability of the experimental results, and the data more objectivity and comparability. The blank group filling of bone marrow blood cells into bone cells, such as a large number of ingredients, but in the absence of new bone growth scaffold, osteogenic effect is far lower than that of other groups. Phrenology comparison with autologous the coral bone hydroxyapatite a better guided bone regeneration ability in early bone growth (2 and 4 weeks) to 6 weeks, the difference was significantly smaller. The low concentration of state fluoride ion can promote the role of bone substitutes guided bone regeneration, accelerate the formation of new bone.
|