Dissertation > Excellent graduate degree dissertation topics show

Combination Use of Bone Tissue Engineering and Guided Bone Regeneration with n-HA/CS/CMC to Repair Segmental Radial Bone Defect in Rabbits

Author: ZhouAiGuo
Tutor: AnHong
School: Chongqing Medical University
Course: Surgery
Keywords: Bone tissue engineering Guided bone regeneration n-HA/CS/CMC Mesenchymal stem cells
CLC: R318.08
Type: PhD thesis
Year: 2009
Downloads: 31
Quote: 0
Read: Download Dissertation

Abstract


ObjectiveTo study the feasibility for repairing segmental defects with single orcombination of n-HA/CS/CMC composite membrane and n-HA/CS/CMCtri-component composite. To provide the rational for the clinical applicationof the new n-HA/CS/CMC composite membrane and n-HA/CS/CMCtri-component composite, and implore the effects on the repairing bonedefects with combined application of guided bone regeneration membraneand tissue engineering bone.MethodsExperiment in vitro: BMSCs were isolated by density gradientcentrifugation from the bone marrow of New Zealand rabbits, and theosteoblasts were differentiated from BMSCs by bone induction. The growthstates and growth curve of BMSCs and osteoblast cells were detected andcompared. The osteoblasts were identified using alkaline phosphatase andcalcium steining. Then, the BMSCs and osteoblasts were inoculated with n-HA/CS/CMC tri-component porous composite and n-HA/PA66by aconcentration of1.0×105/ml, and cultured in vitro. The growth state andadhesion of cells were observed, and the growth and proliferation of cellswere examined by MTT, and the adhesion between cells and material wereobserved by HE staining.Experiment in vivo: The experimental model of10mm bone segmentaldefect were produced in New Zealand white rabbits with both radius, andwere divided into group A, group B and group C according to transplantmaterials. Group A was divided into two groups, the group AL and GroupBL, and the segmental defect on the left side of the former group wasimbedded with n-HA/CS/CMC+BMSCs which was coated with amembrane of n-HA/CS/CMC, while the segmental defect on the right side ofthe latter group was imbedded with n-HA/CS/CMC+osteoblasts, which wascoated with a membrane of n-HA/CS/CMC. Group B: the segmental defecton the left side of the group BL was imbedded withn-HA/CS/CMC+BMSCs+BMSCs without membrane coated, while thesegmental defect on the right side of the group BR was imbedded withn-HA/PA66+BMSCs with a membrane of n-HA/CS/CMC coated. Group C:the segmental defect on the left side of the group CL was coateded with amembrane of n-HA/CS/CMC, while the segmental defect on the right side ofthe group CR taken as the control group was imbedded with nothing.Postoperative gross observation, X-ray evaluation, histological examination and concentration tests of BMP and VEGF in the implanting region wascarried out.ResultsExperiment in vitro: MSCs and osteoblasts presented a good adhesionand growth in the n-HA/CS/CMC tri-component porous composite andn-HA/PA66.Experiment in vivo: according to gross observation, X-ray evaluation,histological examination and concentration tests of BMP and VEGF, newbone formed and increased as time went on in the radial segmental defect inall the rabbits from group A, group B and group CL4weeks postoperatively.In addition, new bone formation developed earlier and the regionalconcentration of the osteoinductive factor was much higher in the group AL,group AR and group BR than group BL and group CL.ConclusionsExperiment in vitro: n-HA/CS/CMC tri-component porous compositecould be considered as ideal carriers for constructing tissue engineering bonein bone defects repairing study.Experiment in vivo: The tissue engineering bone constructed byBMSCs or osteoblasts and n-HA/CS/CMC tri-component porous compositecan be taken as repairing material for the segmental bone defects. Then-HA/CS/CMC composite membrane can guide bone regeneration. And thecombined use of n-HA/CS/CMC n-HA/CS/CMC tri-component porous composite tissue engineering bone and n-HA/CS/CMC membrane canenhance bone defects repairing.

Related Dissertations

  1. Study of Intracerebral Transplantation of Umbilical Cord Blood Mesenchymal Stem Cells in a 6-Hydropamine Rat Model of Pakinson’s Disease,R742.5
  2. The Construction and Evaluation of a Novel Non-viral Gene Transfection System and Its Application in Mesenchymal Stem Cells Gene Recombination,R346
  3. The Protective Effect of Rat Myocardial Ischemia/reperfusion Injury Following Bone Marrow Mesenchymal Stem Cell Pretransplantation for 1 Week,R542.22
  4. The Study of Immuno-tolerance Mechanism of the Third-party Bone Marrow-derived Mesenchymal Stem Cells on Allogenic Transplantation,R392
  5. The Effect of Exogenous Brain-derived Neurotrophic Factor on Transplantation Treatment of Bone Mesenchymal Stem Cells in Intracerebral Hemorrhage in Adult Rats,R743.34
  6. The Research on Treatment of Lentivirus-mediated hVEGF-165 Gene Transferring Mesenchymal Stem Cells on Rat Hind Limb Ischemia,R329
  7. The Research on the Best Time of Bone Marrow Mesenchymal Stem Cell Transplant after Myocardial Infarction,R542.22
  8. Therapeutic Effect and Mechanisms of Derived Mesenchymal Stem Cells in Intracerebral Hemorrhage Rat,R743.34
  9. Effects of Ginsenoside Rg1 on Cell Proliferation, Secretion Function and ERK、p38 Signal Molecular in Human Umbilical Cord Blood Mesenchymal Stem Cells under Optimized Culture Condition,R285.5
  10. Comparison of Chondrogenic Potential between Mesenchymal Stem Cells from Rabbits’ Bone Marrow and Adipose Tissue,R329
  11. Human Adipose Tissue Derived Stem Cells Differentiate into Epidermal Cell and Fibroblasts,R329
  12. The Effects of Trophic Effects of Bone Marrow Mesenchymal Stem Cells on Astrocytes Induced by Carbon Monoxide in Culture,R329
  13. BMP2 and VEGF165 Genes Transfected Rat Bone Marrow Mesechymal Stem Cells Simultaneity Expression,R329
  14. An Experimental Study on the SDF-1/CXCR4 Expression of Infracted Myocardium in Rat after Transplantation of Hypoxia-preconditioned MSCs,R542.22
  15. Effects of Receptor Activity Modifying Protein-1 Transplantation of Mesenchymal Stem Cells on Postangioplasty Restenosis and Cardiac Function after Myocardial Infarction in Rabbits,R542.22
  16. Experimental Investigation of Grafting Pathways of Bone Marrow Stromal Cells into Spinal Cord Injury in Rats.,R651.2
  17. Bone Mesenchymal Stem Cells with Cytosine Deamimase Gene Transducted in Migrat to Glioma in Vivio,R739.4
  18. Four-point bending stress stimulation of human bone marrow mesenchymal stem cell proliferation activity,R329
  19. VEGF-induced neuronal differentiation into mesenchymal stem cells migration research,R329
  20. Autologous bone marrow stem cell transplantation in the treatment of heart failure after myocardial infarction term efficacy,R542.22
  21. Bone marrow mesenchymal stem cells in rats with severe acute pancreatitis CD4 ~ CD25 ~ regulatory T cells influence and Integrative Medicine,R576

CLC: > Medicine, health > Basic Medical > Medical science in general > Biomedical Engineering > General issues > Biomaterial
© 2012 www.DissertationTopic.Net  Mobile