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Background Uncaria tomentosa Rubiaceae Uncaria is the dry cane to sprawling woody plant Uncaria tomentosa Uncaria tomentosa (Willd.) DC, commonly known as the \The plant is native to the tropical rainforests of Central America, Peru, Ecuador, named for its leaf base raw sharp double claw-shaped curved hook. It is reported that the Uncaria tomentosa having immune enhancement, enhancement of DNA repair, inhibition of tumor cell proliferation, inhibition of the neutropenia caused by chemotherapy, anti-forgotten, inflammatory effects. Cat's Claw traditionally used to treat ulcers, rheumatoid arthritis, cancer and the prevention and treatment of leukemia, AIDS. 1970s abroad are carrying out the Cat's Claw chemical composition, pharmacological effects and clinical applications of research confirmed many of the herbal medicine traditional efficacy, and found that the drug can help protect against many incurable diseases troubled modern. The Uncaria tomentosa therefore gradually become the selling herbs in the international market. Uncaria tomentosa and Uncaria same Rubiaceae Uncaria species, its chemical composition is similar to both alkaloids and indole alkaloids. Uncaria tomentosa and domestic the Uncaria chemical ingredients, the similarity, determine their pharmacological effects are similar. Chinese medicine Uncaria as Pinggan wind drugs, its main effect is the role of the inhibitory effect of the central nervous system and lowering blood pressure, the the Uncaria tomentosa domestic Uncaria same central inhibition efficacy and cardiovascular effects? Current Uncaria tomentosa pivotal role and cardiovascular effects have not been reported. The Uncaria tomentosa pharmacological effects in the central nervous system will conduct a preliminary study, and animal models of hypertension on blood pressure observed Uncaria tomentosa explore the mechanisms for the further development and utilization of botanicals experimental basis, and is conducive to the Uncaria plants of the genus Resources Research and Development. Objective To study the Peruvian Uncaria tomentosa the sedative and hypnotic and anticonvulsant effect, lowering blood pressure and related mechanisms, comparison with domestic Uncaria. Method 1. Locomotor activity test: Kunming mice were 50 male and female fasted 12h, drinking water is not limited. The mice were randomly divided into five groups, namely blank control group (saline group of the same capacity), the the domestic Uncaria group (320mg/kg) and Uncaria tomentosa (80mg/kg) in (160mg/kg), high ( 320mg/kg) three dose groups. Measured locomotor activity in mice before administration, the mice in each group into the the YLS-1A multifunctional small animals autonomic activity logger adapt 5 min record time of 5 min. Indoor record when to keep quiet. Ig (ig) and then press the doses given to the mice in each group. Respectively, after administration of 30 min, 60 min each measured once the number of spontaneous activity, recording the number of mice in each group's activities within 5min. Sleep dose of sodium pentobarbital threshold in mice induced sleep test: Take 50 Kunming mice, half male and half female. Grouping ibid, 10 animals in each group. Dose, according to the group of mice ig administered 60 min after the drug to each mouse by intraperitoneal injection of sodium pentobarbital 30 mg / kg, mice turned positive reflex 1min above to fall asleep standard, recorded within 15 min The number of mice in each group go to sleep. 3. Threshold dose of sodium pentobarbital-induced sleep time in mice test: Take 50 Kunming mice, male and female, were randomly divided into 5 groups. Grouping ibid, 10 animals in each group. Dose, according to the group of mice ig administered to each mouse peritoneal injection of pentobarbital sodium 50 mg / kg for 60 min after the drug, and then record the sleep time of mice in each group (in mice after administration turned positive reflex righting reflex time to fall asleep, to restore the sleep time). Strychnine sulfate to mice induced convulsions test: Take 50 Kunming mice were randomly divided into five groups, grouping ibid., n = 10 animals. Ig administered dose, according to the group of mice, 60min, after administration of intraperitoneal injection of sulfate Shi Ning (2mg/kg), record the number of seizures in mice within 2 h after injection of strychnine and the number of dead mice, convulsions reaction to the mouse limb clonic or tonic convulsions indicators. 5 rat hypertension model replication: rats using low-frequency, low-voltage alternating current electric shock plantar combined with noise compound stimulus as a source of stress, the implementation of repeated stress in rats stimulate 28d establishment of stable chronic stress BP animal models. Application of rat tail artery sphygmomanometer repeatedly measured three times, taking the mean systolic blood pressure greater than 18.7Kpa (140mmHg), diastolic blood pressure greater than 12.0Kpa (90mmHg) to model a sign of success, the unsuccessful the animal be removed. Rat model of hypertension experimental groups: the experimental requirements to take blood pressure animals (other than the normal control group), were randomly divided into three groups, 10 in each group, model control group (distilled water) to give the same amount, Uncaria tomentosa group (120mg/kg) and domestic Uncaria group (120 mg / kg), and the other 10 rats a normal control group (distilled water). 7. Rat hypertension model detection indicators: modeling, measuring tail arterial blood pressure of each animal three times in a row after the end of the experiment, the average. After the end of the experiment, the rats abdominal aortic blood 2 ml of injection containing 100 g / L EDTA-Na2 and aprotinin in vitro and mix 4 ° C under the conditions of centrifugal separation of plasma, -20 ℃ and stored. Determination of room temperature before reconstitution, the supernatant was centrifuged again, using radioimmunoassay method for the determination of the animal plasma Ang Ⅱ, ET and CGRP content. Statistical methods: the application SPSS 13.0 statistical software processing data, experimental data (?) ± s between groups using a repeated measures analysis of variance, one-way ANOVA, multiple comparison homogeneity of variance with LSD, arrhythmia with Games -Howell. Count data using χ2 test. P <0.05 indicated that the difference was statistically significant. Results 1. Drugs on the spontaneous activity. The results show that the interaction between time point and group (F = 4.065, P = 0.000); between groups main effect (F = 8.201, P = 0.000); each time point between the main effect (F = 41.712, P = 0.000). No change in the number of spontaneous activity before and after the administration of the blank control group (P> 0.05). 30 minutes after the drug reduced the spontaneous activities of the domestic the Uncaria group mice, compared with before administration, the differences significant (P <0.01), compared with the blank control group, also a significant difference (P <0.01); Uncaria tomentosa , medium, and high dose groups of mice did not alter the locomotor activity, compared to before administration and compared with the blank control group, there was no difference (P> 0.05). Uncaria tomentosa low, medium, and high dose group the small rat poison after 30min, the number of independent activity and domestic the Uncaria group comparison, there is a significant difference (P <0.01). Drug 60min, the domestic of Uncaria group and Uncaria tomentosa low, medium, and high dose groups of mice were reduced spontaneous activity, compared with before administration, a significant difference (P <0.05); compared with the blank control group, there are significant difference (P <0.01); the Uncaria tomentosa low, medium, and high dose group compared with domestic Uncaria group, there was no significant difference (P> 0.05). 2 doses of drugs on the threshold of sodium pentobarbital sleep in mice the sleep the impact of the number of: blank control group, intraperitoneal injection of subliminal sleep dose of sodium pentobarbital (30 mg / kg), turned positive reflex mice for three. The domestic of Uncaria group and fluff the Uncaria low dose group have eight mice sleep, Uncaria tomentosa, each nine mice in the high dose group sleep. The number of mice to sleep after x ~ 2 test x to 2 = 13.098, v = 4, P = 0.011 (two-sided), the difference was statistically significant, can be considered to have significant differences between the five experimental groups Uncaria tomentosa, sleep number up to the high dose group. 3. Drug sleep dose of sodium pentobarbital threshold in mice sleep time: the mice sleep time data via one-way ANOVA analysis showed that the group has a significant difference (F = 24.304, P = 0.000) need further pairwise comparisons (LSD method). Results: blank control group Uncaria tomentosa low dose group no significant difference (P = 0.379) were significantly different (P <0.01), compared with other groups. Domestic of Uncaria group with Uncaria tomentosa high-dose group showed no significant difference (P = 0.343), a significant difference compared with other groups. (P <0.05). The results show that the threshold dose of sodium pentobarbital domestic Uncaria Uncaria tomentosa administration can extend the duration of the mice of sleep caused by the Uncaria tomentosa role with the dose increase and strengthen. 4. Drug Strychnine sulfate convulsion in mice: mice by intraperitoneal injection of strychnine (2mg/kg), mice and more within 10 min tonic convulsions and death. The number of seizures in mice by x ~ 2 test results show that the x to 2 = 2.500, v = 4, P = 0.645 (two-sided), the difference was not statistically significant, can be considered small among the five experimental groups the mouse convulsion number was no significant difference. Blank control group were killed 8 mice. Domestic the Uncaria groups have two mice died, Uncaria tomentosa, dead mice in the high-dose group, respectively, for 6,4,2. Number of mice died after x ~ 2 test results show that the x to 2 = 11.303, v = 4, P = 0.023 (two-sided), the difference was significant that the differences between the five experimental groups significantly significance, the least number of death of which the the domestic of Uncaria group and Uncaria tomentosa high dose group. 5. Drugs on the blood pressure of rat model. Systolic blood pressure: between time point and group interaction (F = 16.240, P = 0.000); between groups main effect (F = 9.951, P = 0.000); each time point between the main effect (F = 98.796, P = 0.000), at each time point using one-way ANOVA, between groups using the Total point in time the main effect of repeated measures analysis of variance. Modeling, systolic blood pressure among the groups were not significantly different (P> 0.05). Systolic blood pressure after modeling, model control group, the group of Uncaria tomentosa and the domestic Uncaria group and normal control group has a significant difference (P = 0.000) between the three groups, no significant difference (P> 0.05). Systolic blood pressure after treatment the Uncaria tomentosa group the domestic Gouteng group and normal control group and model control group, there were significant difference (P = 0.000) among the three groups, no significant difference (P> 0.05). Diastolic blood pressure effects: between the time point and group interaction (F = 3.388, P = 0.009); between groups main effect (F = 1.634, P = 0.199); each time point between the main effect (F = 17.661, P = 0.000), at each time point using one-way ANOVA, between groups using the Total point in time the main effect of repeated measures analysis of variance. Modeling diastolic blood pressure among the groups were not significantly different (P> 0.05). Modeling and diastolic blood pressure, model control group (P = 0.028), the Uncaria tomentosa groups (P = 0.027) and the domestic the Uncaria group (P = 0.014) and normal control group had significant differences between the three groups significant difference (P> 0.05). Diastolic blood pressure after treatment, normal control group (P = 0.014), of Uncaria tomentosa group (P = 0.013), and domestic Uncaria group (P = 0.008) and the model control group, there were significant differences among the three groups, no significant difference (P> 0.05). In short, the establishment of the first three groups of animals in the model of blood pressure were statistically differences no statistically significant (P> 0.05), modeling, animal blood pressure were significantly increased (P <0.05) after 7 days of treatment, no blood pressure of model animals significant change (P> 0.05); of Uncaria tomentosa groups and domestic of Uncaria group of blood pressure decreased significantly (P <0.01). Between the two groups, the two-dose group compared with model group significant difference (P <0.05), but the two-dose group group no significant difference (P> 0.05). Drugs hypertensive rat plasma Ang Ⅱ, ET and CGRP: model group, Ang Ⅱ and ET levels increased, compared with the normal control group and domestic Uncaria Uncaria tomentosa group, there is a significant difference (P <0.05) . Model group, CGRP levels, and domestic Gouteng group, the the Uncaria tomentosa group and the normal control group compared significant difference (P <0.01). Conclusion 1. Uncaria tomentosa ethanol extract can significantly reduce locomotor activity of mice, the increase turned positive reflex mice, prolong sleep time. Domestic Uncaria Uncaria tomentosa sedative and hypnotic effects. Uncaria tomentosa can reduce the number of strychnine convulsion caused by the death of mice, but no obvious effect on mice the number of seizures. The Uncaria tomentosa anticonvulsant effect similar to the domestic Uncaria. 3 the Uncaria tomentosa and domestic Gouteng of the same antihypertensive effect, its blood pressure lowering effect may be related to the regulation of the renin - angiotensin - aldosterone system.
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