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Purpose according to traditional Chinese medicine theory and application background to single herbs A to charcoal hemostasis entry point for the study, through medicinal chemistry and pharmacology two aspects to explore A charcoal essence of hemostasis and tried to reveal the A before and after processing for the treatment of bleeding disorders markedly part of the mechanism. Method 1. Traits in processed products, extract content and pharmacodynamic basis for the comprehensive evaluation, using Orthogonal A drug processing technology to charcoal. 2 color by chemical reaction A charcoal to fry around flavonoids qualitative inspection knowledge and the spectrophotometric determination of total flavonoids. 3 using inductively coupled plasma atomic emission spectrometry (ICP-AES) to determine A crude drug, carbon drugs and their water extract closely related with the hemostatic effects of inorganic elements K, Ca, Mg, Zn, Fe, Mn, Cu , Al, and calculate the dissolution rate of the elements. 4 experiments using mice tail bleeding and clotting capillary glass tube experiments to observe the A bleeding time of mice before and after processing (bleeding time, BT), clotting time (clotting time, CT) and the anticoagulant effect of heparin. 5 using rabbit experimental traumatic bleeding and clotting test tube experiments, observed in rabbits before and after processing A to BT, CT effect. 6 using solidification method, turbidimetric method, in vitro determination of rabbits before and after administration of prothrombin time (prothrombin time, PT), activated partial thromboplastin time (activated partialthormboplastia time, APTT), thrombin time (thrombin time, TT), fibrinogen (fibrinogen, FIB), plasma recalcification time (plasma recalcificationtime, PRT), euglobulin lysis time (euglobulin lysis time, ELT), maximum platelet aggregation rate (max platelet aggregation rate, MPAR), Analysis A to rabbits before and after processing coagulation, fibrinolysis and platelet function. Results 1. Variance analysis showed that A to fry their quality charcoal cooked temperature has a significant effect (P <0.05), poor analysis showed that frying time on the A to the quality of processed products also have some impact. (2) A crude drug to the type of flavonoids in flavonoids, flavonols based, and charcoal pills to chalcones and isoflavones based. Crude drugs and drug charcoal total flavonoids were 4.78 percent and 2.26 percent. 3 A for crude drugs and charcoal pills eight kinds of inorganic elements in the level of K> Ca> Mg> Al> Fe> Mn> Zn> Cu; crude drug by fried carbon concocted after eight kinds of inorganic elements content in varying degrees increasing, but the rate of dissolution in water element significantly reduced. 4 A to mice before and after processing BT, CT effects: charcoal pills, high-dose group could significantly shorten the mouse BT (P <0.01); crude drug high dose group and charcoal pills, high-dose group could significantly reduced in mice CT (P <0.01). 5 A for processing before and after heparin Effect: raw drugs and charcoal drug group could significantly shorten heparinized mouse BT, CT (P <0.01). 6 A to rabbits before and after processing BT, CT effects: crude drug, the high dose group and carbon drugs low, medium and high dose groups could significantly shorten the rabbit BT (P <0.01 or P <0.05); crude drugs and Carbon drugs each dose group could significantly shorten rabbit CT (P <0.01 or P <0.05). 7 A given before and after processing of rabbit coagulation system: raw drugs and charcoal medicine each dose group the PT had no significant effect (P> 0.05); crude drug high dose group and carbon drugs low, medium and high dose group was significantly shorter APTT (P <0.01 or P <0.05); crude drug high dose group and high dose group carbon drugs can significantly shorten the TT (P <0.01 or P <0.05); charcoal pills, high dose group significantly increased FIB (P < 0.01 or P <0.05); charcoal drug high dose group can significantly shorten the PRT (P <0.05). 8 A to rabbits before and after processing the fibrinolytic system and platelet function: crude drug drugs high dose group and char low, medium and high dose groups could significantly prolong the ELT (P <0.01 or P <0.05); carbon high-dose drug group could significantly increase the MPAR (P <0.01). Conclusions 1. A processing technology for the best charcoal is 210 ℃ frying 20 min. (2) A to flavonoids as the main chemical ingredient. After processing, change the type of flavonoids and total flavonoids content was significantly reduced. 3 A to crude drugs by processing, the inorganic element content increased, but the dissolution rate was significantly reduced. 4 A crude drugs to have a good hemostasis, procoagulant effect, concocted after effects have enhanced trend. 5 A to crude drug through the influence of the intrinsic coagulation pathway, the fibrinolytic system to play its role in hemostasis procoagulant, concocted after the effect has increasing trend, and the drug can significantly increase carbon FIB and MPAR, more conducive to promoting blood solidification.
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