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Study on the Effect of Stretching to Fascial Remodeling and Different Transcription Level of Remodeling-Related Gene

Author: ZhangXueQuan
Tutor: YuanLin
School: Southern Medical University,
Course: Human Anatomy
Keywords: Fascia school Connective tissue Stretch stimulus Cell proliferation CTGF TGF-β1 Procollagen-1 Procollagen-3 MMP-2 MMP-3 TIMP-1 TIMP-2
CLC: R245
Type: Master's thesis
Year: 2009
Downloads: 60
Quote: 0
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Abstract

Background: traditional Chinese medicine as a crystallization of China's thousands of years of culture, plays an important role in the up and down in the transmission of Chinese civilization, the people to prevent and cure diseases, in particular an integral part of preventive health care process technology developed era, confirmed by the practice of the ancient Chinese literati Patriots Experience this won the Volkswagen respected. In recent years, with the development of science and technology, cultural exchanges between the acceleration and the people of the green treatment, the treatment carried out under the guidance of TCM theory, not only in the Chinese residential area but also in Europe and the United States developed regions such as the United Kingdom, Germany, France, Belgium, the Netherlands Canada, the United States, is widely used as a supplement or alternative treatment undertaken. And as one of the core of traditional Chinese medicine meridian theory, or under the guidance of the theory of acupuncture practice, due to the majority of scholars it is green means and mechanisms mystique to attract domestic and foreign-depth study. Recalling the history of Acupuncture and Meridian and abroad to form the two periods of the fifties and sixties of the twentieth century, and since the end of the twentieth century, China recently invested a lot of money and human resources to carry out a comprehensive multi-disciplinary research and climbing, after more than fifty year three sections of the peak period, the establishment of the Eighth Five-Year Plan \correlation, and further found that the The Meridian has cross-somite conductivity, follow via two-way transitivity, low resistance and high conductivity, high for Luminescence sexual calcium enrichment sexual characteristics. Looking for decades meridian Quest various hypotheses to pour contentious, difficult to convergence. The biggest rift than \The meridian research after the storm, and gradually form the neurophysiology to send - nerve conduction doctrine, physiological and biochemical School - fluid circulation theory, the biophysics sent - biological field theory, but these doctrines uncompromising confirmed the existence of the meridian, and only from one side or angle all but never won. Nearly from the original Professor connective tissue in the human body the linkages \and signal network view, the researchers gradually recognize this structure in the importance of the human body, is worth mentioning is the live forest Prof. bear the National 863 Program \fascia bracket on human connective tissue composed of markers and three-dimensional computer reconstruction database system project process, drawn after the fascia in the human body from the shallow into the deep by five kinds of structure: 1, dermis dense connective tissue; 2 subcutaneous loose connective tissue; 3, the surface of the muscle loose connective tissue; septum and muscle gap connective tissue;, internal organs door envelope and internal partitions of connective tissue. Task Force found by studying the GB body 14 meridians 361 acupoints needle position and practices of human acupuncture points acupuncture sites are located at different levels of the fascia, intervals and muscle gap connective tissue in the muscle, followed by dermal dense connective tissue layer and subcutaneous loose connective tissue layer, a few in the muscles the surface loose connective tissue (deep fascia) and internal organs, doors, envelope and internal partitions of connective tissue. Former Professor Lin research group through the study and combine developmental biology proposed: mesenchymal mesoderm to differentiate into multiple organ systems constitute the remaining part of the the fascia bracket \The stent cell body, to the fibrous framework, the extracellular matrix construct throughout the body space to communicate with each other \cell reserve and support for simultaneous detection of changes in the environment and regulation of the body, the new perspectives that support Reserve System. The view that the subcutaneous fascia tissue in the body's internal plays an important role of the media in response to stimulation or exogenous stretch and play a regulatory role, and to speculate the various replacement therapy (supplement therapy, mainly referring to in traditional Chinese medicine acupuncture, massage and physical therapy measures carried out under the guidance of the theory, the study confirmed that these treatments mesh fascia bracket play the role of biological effects) target precisely the body primarily through the fascia connective tissue deformation under stress stimuli. The research group in early experiments by means of ultrasound, CT, MRI and other medical imaging and China Digital massive data sets that: in the process of acupuncture, by lifting and thrusting, twisting and other operations, can drive or affect the surrounding larger range connective tissue. \and CT images to build the connective tissue fascia bracket is more consistent with the human body the classic meridians go line and get the MRI image confirmed; research group confirmed the fascia bracket with classical meridian line consistency while also recognize the existence and classic diameters Running inconsistent structure control TCM acupuncture points record: Chinese medicine believes that the human body both Meridian Ashi points, Meridian Meridian Distribution A hole distribution is no fixed position, which put forward the body's connective tissue scaffold Anatomy of Acupuncture and Meridian material basis; acupuncture points in acupuncture in the connective tissue holder to mention plug and twisting can produce strong biological information site; acupoints and non-acupoints only differences in the amount of information and there is no qualitative difference; generalized speak in various parts of the body are acupuncture points, because the gap between almost all the body's tissues and organs have connective tissue. The points of the ancient records is human connective tissue gathered at stimulating these areas through its own neural regulation and humoral regulation play efficacy. However, the research group in the previous study confirmed the existence of the fascial network rack, multi-focus imaging or gross morphology fascia connective tissue fiber structure and mechanical stimulation of the cells of the basic unit of life activities how to react, life adjust the basic form of gene regulation is involved in this process to study very little, which is also discussed in this study. In this study, by means of immunocytochemistry and molecular biology methods discuss stretch stimulation of the fascia and connective tissue remodeling and gene transcription levels from the microscopic point of view, the material foundation of meridian, and provide theoretical support for the fascia doctrine. Purpose fascia connective tissue remodeling and gene transcription level by studying the stretch to stimulate further provide evidence to support Meridian and connective tissue, fascia school provides theoretical support, and explore a variety of alternative clinical therapy treatment mechanism. 1, through the study of the impact on the the stretch stimulation fascia connective tissue fiber morphology of collagen fibers or elastic fibers stretch stimulus. By the stretch stimulation fascia connective tissue cell morphology impact of research, to discuss the possible biological behavior of the cells within the fascia connective tissue in the feel of the tensile stress. 3, through the study of the influence of the level of gene transcription stretch stimulation fascia connective tissue remodeling discuss the the stretch stimulation possible biological significance. Method 1, with the Chinese medicine acupuncture methods applied tensile stimulate cut subcutaneous loose connective tissue and stretch stimulate fiber Traveling observed by phase contrast microscopy. 2, subcutaneous loose connective tissue, cut stretch stimulation by the phalloidin / Pi dyeing, by fluorescence microscopy tensile stimulate fascia connective tissue cell morphology. By RT-PCR method to study the stretching stimulus impact on the level of gene transcription fascia connective tissue remodeling: of CTGF, TGF-β1, Procollagen-l (Procollagen-3), MMP-2, MMP-3, TIMP- 1, TIMP-2. 1, stretching stimulate connective tissue fiber orientation and its direction of force, twisting acupuncture can drive the connective tissue displacement within a certain range. 2, the change of the arrangement of fibers and connective tissue displacement caused by connective tissue cells sense mechanical stimulation, the cells reconfigurable growth fusiform, the same direction of the long axis direction cells by force. 3 cells feel the stretch stimulated CTGF, TGF-β1, TIMP-1 transcription level downward adjustment (F values ??for 4.774,6.151,15.877 and p values ??were 0.023,0.011,0.000) while Procollagen-1 Procollagen-3, MMP-2, MMP-3, TIMP-2 had no effect (F values ??were 0.369,0.252,2.466,1.608,1.440, the P values ??0.695,0.779,0.115,0.219,0.265). Conclusion: acupuncture methods applied tensile stimulation needle twisting collagen fibers or elastic fibers by electrostatic interaction between the needle wound in the needle body stretch stimulation can drive around fiber movement so that the connective tissue deformation occurs. Fiber to maintain its original disordered arrangement of the control group. 2, tensile stimulus driven fiber deformation, mechanical stimulation signal through the fiber conduction the plaques composite structure into the cell-cell adhesion, thus causing the cytoskeleton remodeling, fusiform cells. 3, stretching stimulate gene transcription level CTGF/TGF-β1/TIMP-1 regulation may be one of the mechanisms of alternative therapies play a therapeutic role. In short, with the method of acupuncture twisting stretch stimulus is applied, electrostatic coupling effect between the connective tissue fibers through the needle body is wound around the needle body twisting tension directly contributing to the connective tissue fibers causing connective tissue deformation, connective a direct result of tissue deformation is driven with the fibers through contact vinculin composite cells (mainly to fiber cells, there are a few mesenchymal stem cells, etc.), thereby stretching stimulation signal to be delivered to cells in the intracellular cytoskeleton remodeling as well as a series of signaling cascades exogenous stretch stimulatory signals delivered to the nucleus, causing a series of changes, including the regulation of cell proliferation and the level of gene transcription. Fibroblasts as connective tissue cells involved in the body damage and repair process, while the synthesis of a variety of cytokines, including matrix formation or degradation factor. Matrix formation or degradation directly affect the permeability of the basement membrane, which has a close relationship with a variety of diseases long hair; mesenchymal stem cells as a pluripotent stem cell differentiation to multi-directional and from a number of supplementary necrosis or loss of function of cells involved in injury repair and reconstruction; stretch stimuli delivered to the nucleus, causing a series of gene transcription level differences such as CTGF/TGF-β1/TIMP-1, these genes confirmed also the repair and reconstruction of the organization plays an important role, and regulate the transcriptional activity of the downstream factors, so stretch stimulation fascia connective tissue deformation may be just what the alternative therapies pathway of cell activity or gene transcription The level of regulation may be just what the mechanism of action of clinical replacement therapy.

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