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Gerbera varieties ' pretty pink ' , brightly colored squid upright , cut flowers is high, long shelf life , and has a very high ornamental value . Gerbera using rapid propagation in vitro culture methods , not only to the large population in a short period of time good seed , but also avoid varieties degradation brought about by the by division propagation and seed multiplication ; in Gerbera growth process vulnerable to virus hazards seriously affect the quality and yield of cut Gerbera flower . The purpose of this pilot study is ' pretty pink ' from the two aspects of the problem body culture and detoxification technology , is important to maintain a ' pretty pink ' excellent germplasm resources with excellent characteristics of stability , enhance its ornamental value . 1 to no pests ' pretty pink ' mother plant for materials, select the receptacle, shoot tips , petioles as explants , MS medium , additional different concentrations of 6- BA of NAA, induced explants produce callus organization , followed by differentiation budding ; rooting and rooting medium ; after transplanting tissue culture in different matrix , selection of the most suitable culture medium . 2 on greenhouse production plants and receptacle tissue culture explants of tomato black ring virus ( Tomato black the ring virus TBRV ) , tobacco rattle virus ( Tobacco rattle virus TRV ) , cucumber mosaic virus ( Cucumber mosaic virus, CMV), tobacco mosaic virus (Tobacco mosaic virus, TMV), tomato spotted wilt virus (Tomato spotted wilt virus, TSWV) 5 gerbera susceptible to the virus by RT-PCR results showed that the two sources material only carry tobacco mosaic virus . 3 ' pretty pink ' tissue culture for the material, the shoot apex organization detoxification , shoot tips ribavirin ( triazole nucleoside ) processing detoxification Tori organization ribavirin treatment detoxification , heat treatment detoxification , ribavirin off heat treatment drug detoxification treatment . Treated plantlets were detected by RT-PCR , the test results show , the shoot apex organizations the detoxification and shoot tips ribavirin ( ribavirin ) processing detoxification of Gerbera ' pretty pink ' detoxification reach 100 % receptacle tissue ribavirin 5mg / L medium plantlets detoxification treatment rate reached 85% , after the heat treatment , tissue culture showed adaptability .
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