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Space breeding of of Camptotheca acuminata endophytic fungi anabolic growth and development characteristics

Author: ZhangZuo
Tutor: LiuJiaJia
School: Central South University
Course: Pharmaceutical Engineering
Keywords: Camptothecin Endophytic fungi Space Breeding
CLC: S567.19
Type: Master's thesis
Year: 2011
Downloads: 26
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Abstract


C. acuminata dogwood mesh Davidia involucrata Kodjo wood plant is endemic to China a deciduous tree , widely distributed in the Yangtze River basin and southwest provinces . In 1966 , the first time for the United States the Monroe E.Wall of from Camptotheca acuminata stem extract isolated camptothecin , then people found camptothecin have a certain effect on gastrointestinal cancer, bladder cancer , liver cancer and leukemia and other malignancies . The Camptothecin the unique anticancer activity mechanism set off the climax of Camptotheca acuminata with camptothecin . This paper carried out the separation of the endophytic fungi of Hunan Zhangjiajie Camptotheca acuminata , screening and product anticancer properties . The purpose of looking for the broader sources of camptothecin , to effectively produce camptothecin substances target new strains , while the development of new natural medicines , lay the final production of the drug through microbial engineering to solve some of the key issues in the camptothecin production and research base. Isolated endophytic fungi in the bark of this article from Hunan hi , through the liquid medium fermentation after 15 Camptotheca acuminata strains isolated by thin-layer chromatography method ( TLC ) , high performance liquid chromatography (HPLC) detection found an extracellular secretions containing camptothecin, numeral EFC of I - 03 , this strain has a further value of the study . Strains EFC I - 03 space breeding , research space breeding Camptotheca acuminata endophytic fungi physiological characteristics and camptothecin production capacity . The test results found that strains with fast growth advantage in the first generation of space mutagenic effects , but this advantage is not having hereditary , second and third generation space strains cultured in growth rate and ground control strains growth speed basic synchronization . Space strain and accepted the mutagenic effects of space is no significant increase in the production of camptothecin ability . Added to the culture medium Camptotheca acuminata endophytic fungi KH2PO4.MgSO4 and ( NH4 ) 2SO4 three kinds of salt, three factors and three levels of orthogonal test optimization . The test results show that the effect of optimization of the culture medium using 4 program is preferably highest camptothecin yields can be obtained .

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CLC: > Agricultural Sciences > Crop > Economic crops > Medicinal crops > Woody > Other
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