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Studies on the Separation of Rapeseed Protein and Rapeseed Oil by the Technology of Reversed Micelles Extraction

Author: ChenYinHe
Tutor: ZhengJingCheng
School: Wuhan Polytechnic University
Course: Cereals, Oils and Vegetable Protein Engineering
Keywords: Reverse micelles Rapeseed protein Rapeseed oils and fats Features
CLC: TS229
Type: Master's thesis
Year: 2009
Downloads: 193
Quote: 2
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Abstract


Reverse micelles extraction is a new type of biomolecule separation technology. The application of reverse micelles from vegetable oil compound extraction separation of proteins and fats, can not only shorten the traditional process is complex and lengthy process, and can avoid the drawbacks of traditional separation methods easily denatured protein. By comparing the two surfactants (SDS and CTAB) and co-solvent (n-octanol and n-butanol) ability to form reverse micelle system and extracted protein obtained CTAB / isooctane / octanol reverse micelles The system is more suitable for the extraction separation of rapeseed protein and fats; This reverse micelle system and preliminary research forward extraction and stripping process, rapeseed protein and fat; analysis of canola oil and protein extraction separation and determination product comparison with traditional methods, to provide a theoretical basis for further research and application of reverse micelles extraction technology. For forward extraction process, through single factor test investigated the impact of various factors (extraction time, the extraction temperature, surfactant concentration, W0, extraction pH, ion concentration, the ratio of surfactant and co-solvent) for protein extraction rate before . Combined with orthogonal experiment, to the protein forward extraction rate as the index, derived best before the extraction process conditions: raw material particle size of 60 mesh, the amount of raw materials to add 0.04g/ml, w0 for 30 the CTAB concentration 0.06g/ml Isooctyl alkoxy and n-octanol the volume ratio of 5 and a pH of 7.0, of KCl concentration of 0.07mol / L, the extraction temperature is 45 ° C, the extraction time of 110min. Analysis of variance showed that the active agent have a very significant effect on the concentration of the protein before extraction rate. Stripping process, through the response surface experiment analysis show that: The single factor A (the pH value), B (ion concentration), C (stripping time) are stripping rates have significantly affected, and the impact of the order to: A gt; C gt; B. The interactions between the three factors are not significant. Comprehensive consideration, and combining software analysis of the derived optimal stripping process conditions as follows: pH 8.0, and the ion concentration of 1.0 mol / L, and the stripping time 80min. After stripping, the organic phase according to the physical properties of the ethanol extraction and rotary evaporation method of combining separated rapeseed oil, the fatty acid composition, compared with the n-hexane extraction method resulting rapeseed fatty acid composition almost no change and analyzed by gas chromatography. After stripping of the protein phase after dialysis and freeze-dried to obtain a high purity protein powder, the use of high performance liquid chromatography analysis of its amino acid composition compared to the composition with the alkali-soluble and acid precipitation of the protein amino acids, glutamic acid, lysine, refined acid content extracted more than 30% higher than the alkali-soluble and acid precipitation, aspartic acid, threonine, serine, proline, valine, tyrosine, phenylalanine content than the alkali-soluble acid Shen Extraction of more than 30% of the low, not very different from the rest of the amino acid content. Know by SDS-PAGE electrophoresis pattern BandScan software for protein analysis, reverse micelles extraction protein subunit molecular weight 14kDa about 60% the 25kDa less than 80%. Compare reverse micelles and alkali solution and acid protein features known the reverse micelles protein solubility, foaming, emulsification Jiaoyou; water holding capacity, foaming stability is poor.

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