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Objective To observe the processing levocarnitine ischemic period of the protective effect of myocardial ischemia - reperfusion injury in the rat ; explore the ATP-sensitive potassium channels (KATP) is involved in the protective effect of L-carnitine on cardiac myocytes . Methods 30 SD rats were randomly divided into three groups , ischemia-reperfusion group (I / R group ) , levocarnitine treatment group (L-CN group ) , glibenclamide levocarnitine treatment group (Gli L-CN group ) , 10 in each group . I / R group , MI 5 - minute intravenous infusion of normal saline to the end of the experiment , the drip rate 0.5ml/min ; L -CN group , MI 5 minutes after levocarnitine 3.0g adding saline 250ml intravenous drip to experiment end ; Gli L-CN group , MI given five minutes of L-carnitine 3.0g normal saline 250ml while adding 10μmol / L glibenclamide intravenous infusion to the end of the experiment . Was extracted from each group at the end of reperfusion arterial 3ml 2000r/min centrifugal 10min , the supernatant was measured by ELISA of cTnI colorimetric determination of serum CK and LDH activity . Take apical myocardial tissue , 10% formaldehyde solution fixed , paraffin-embedded light microscope to observe the degree of myocardial damage . Compared with the I / R group , L - CN group cTnI, LDH, CK decreased ( P lt; 0.05 ) ; compared with the L-CN group , the Gli L - CN group cTnI, LDH, CK increased (P lt; 0.05). Conclusion L-carnitine on myocardial ischemia and reperfusion protective effect may be related to activation of ATP-sensitive potassium channels .
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