Dissertation > Excellent graduate degree dissertation topics show

Propranolol for Haemangiomas-insight into VEGF/ERK Signaling Transduction in HUVEC-12 Cells

Author: LiSuo
Tutor: LuJianYun
School: Central South University
Course: Dermatology and Venereology
Keywords: propranolol haemangiomas endothelial cell vascular endothelial growth factor (VEGF) ERK signaling pathway
CLC: R965
Type: Master's thesis
Year: 2011
Downloads: 70
Quote: 0
Read: Download Dissertation

Abstract


Objectives1. To investigate the influence of Propranolol on the the cytotoxicity and proliferation of the endothelial cells in vitro.2. To investigate the action of propranolol involved haemangiomas related to VEGF/ERK signaling transduction (ERK:extracellular signal related kinases) in cultured human umbilical vein endothelial-12 cells (HUVEC-12 cells)Methods1. Endothelial cells were treated with different dose of propranolol, the cell morphologcal changes were observed by light microscopy; the cell proliferation activity and cell viability was detected by MTT assay; p-ERK1/2 expressions were assessed by western bloting.2. Endothelial cells were treated with different dose of propranolol, p-ERK1/2 expressions were assessed by western blot.3. Endothelial cells induced by VEGF were treated with different dose of propranolol, the cell proliferation activity and cell viability was detected by MTT assay, p-ERK1/2 expressions were assessed by western bloting.4.In the study of intervention, endothelial cells induced or not by VEGF were treated by PD98059 (specific inhibitor of ERK) and/or propranolol, the cell proliferation activity and cell viability was detected by MTT assay, p-ERK1/2 expressions were assessed by western bloting.Results1. propranolol can slight activated proliferation of endothelial cells(P<0.05) and it has no impacts on expression of p-ERK1/2 and cell viability(P>0.05).2. The VEGF positive system of endothelial cells was estabished when VEGF is of 20ng/ml,in which the p-ERK1/2 is highest expression(P<0.05).3.The proliferation and the expression of p-ERK1/2 of endothelial cells induced by VEGF were inhibited by propranolol at the concentration between 8-16 ug/ml (P<0.05),and cell viability have no significant changes(P>0.05)4. Cell viability and proliferation have no significant changes when PD98059 at the concentrtion of 12.5umol/l,and PD98059 at this concentration can inhibited the expression of p-ERK1/2 and cell proliferation induced by VEGF.And we also find that propranolol has the same effect as PD98059,and its efficacy may be of synergy.ConclusionThe mechanism of propranalol control the growth of infantile haemangiomas may be due to inhibite the proliferation of EC induced by VEGF, for it has specific negative effect on ERK pathway.

Related Dissertations

  1. Effects of Fix 6,7-furocoumarins on the Hepatic Microsome Cytochrome P2C9, 2C19 Activity in Rats,R965
  2. Influence and Significance of MMP-2 and VEGF of Hemangioma Endothelial Cells in Vitro Culture by Propranolol,R739.5
  3. The Analysis of the Growth Factor Related with the Secondary Glaucoma in the Anterior Champer of Silicone Oil-filled Eyes,R779.6
  4. Intense Pulsed Light in Effects of TGF-β1 Induced on the Proliferation of Fibroblasts and Intervention of ERK Inhibitor,R751
  5. Comperative Experiment on the Toxicity of Particulate Matter from Different Cities,R114
  6. Propranolol Hci Slow-release Microsphere Preparation,R94
  7. The Study of the Clinical Observation and the Mechanism of Infantile Hemangioma Treatment with Propranolol,R969
  8. Mechanism of Propranolol-induced Apoptosis in Human Umbilical Vein Endothelial Cells,R96
  9. The Polymorphism of Sites 373 and 1688 of PECAM-1 Gene in Mucocutaneous Lymph Node Syndrome in Children,R758.6
  10. pEGFP/Ang-1 Transfected BMSCs Attenuates High Glucose Concentration-induced Choroido-retinal VECs Damage,R774.1
  11. Correlation between CT Perfusion Imaging in Nonsmall-cell Lung Cancer and VEGF、MVD、VEGF-C、LVD,R734.2
  12. Relaionship among Expression of ESM-1, VEGF-D, Lymphangiogenesis and Lymph Node Metastasis in Human Invasive Lobular Breast Cancer,R737.9
  13. Changes in the Expression of Transforming Growth Factor Alpha and Epidermal Growth Factor Receptor on Portal Hypertensive Gastropathy Healing in Patients,R575.2
  14. PECAM-1 Gene Polymorphisms in the Exons 3 and 8 with Coronary Heart Disease in the Han of Western Liaoning,R541.4
  15. Effect of Continuous Blood Purification on Prognosis and Endothelial Cell Function in Patients with Severe Sepsis,R459.7
  16. The Expression of the Angiopoietin and Vascular Endothelial Growth Factor (VEGF) in Cervical Cancer and the Relationship with the Neogenetic Blood Vessel Density and Lymphatic Density,R737.33
  17. The Relationship between the Expression of Signal Transducers and Acti-vators of Transcription STAT3 and Vascular Endothelial Growth Factor Expression and the Clinical Biological Behavior in Gastric Cancer,R735.2
  18. TP on the AIA rat VEGF, FLT1 and CCR5 expression,R684
  19. Effects of Clenbuterol & Propranolol on Bone Metabolism in Ovariectomized Rats,R580
  20. Effects of VEGF Toward to Formation of Vulnerable Atherosclerotic Plaque Interfered by Statins,R541.4

CLC: > Medicine, health > Pharmacy > Pharmacology > Experimental Pharmacology
© 2012 www.DissertationTopic.Net  Mobile