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Preparation of Doxorubicin Hydrochloride Nanoliposome and Its in Vitro Antitumor Activity

Author: WeiLiZuo
Tutor: ChenYuXiang
School: Central South University
Course: Biochemistry and Molecular Biology
Keywords: Doxorubicin hydrochloride Nanoliposome Prescription process Antitumor Cell morphology
CLC: R96
Type: Master's thesis
Year: 2011
Downloads: 234
Quote: 1
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Abstract


Objective: To optimize the doxorubicin hydrochloride nano liposome preparation methods and prescription process, carried out detailed research into its anti-tumor effects in vitro and anti-tumor effect of free doxorubicin hydrochloride solution for systematic comparison, for the The clinical trials of the product and the further development and utilization of early experimental lay a good foundation and conditions for. Methods: 1, In this study, the selection of a certain proportion of egg yolk lecithin and cholesterol mixture wrapped doxorubicin hydrochloride liposome, prescription Twain -80 increase of doxorubicin hydrochloride nanoliposome (DONP-HCL) dissolved ; use of vitamin E, as stabilizing agent, to reduce the oxidation of the egg yolk lecithin, to reduce the leakage rate of the drug to improve drug stability. At the same time, we have adopted a new ethanol injection-PH gradient - motor agitation preparation to improve the drug encapsulation efficiency, control of the particle size of the nano-liposomes. Select hepatoma cells HepG-2, breast cancer cells MCF-7, cervical carcinoma of Hela, gastric cancer cell SGC glioma cells U251 five kinds of cell lines, the doxorubicin hydrochloride nano prepared by the MTT assay fat plastids (DONP-HCL) on various tumor cell killing ability. 3, acridine acridine orange (AO) and ethidium bromide (EB) cells fluorescent double staining observed the doxorubicin hydrochloride nanoliposome drugs on a variety of tumor cell apoptosis morphology. Results: prepared in this experiment new uniform particle size distribution of liposomal doxorubicin nano control in between 140 ~ 170nm, encapsulation efficiency up to 99.85%, and stored at low temperature relatively stable; MTT test results show that the experimental preparation hydrochloric acid A the neomycin nanoliposome (DONP-HCL) the hepatoma cell HepG-2, breast cancer cells MCF-7, cervical cancer Hela, gastric cancer cell SGC glioma U251 cells IC50, respectively 0.055μ / the mL, 0.8717μg/mL1.2256μg/mL, 1.5601μg/mL, 1.6061μg/mL, while the same concentration of free doxorubicin hydrochloride solution IC50 of various tumor cells 0.932μg/mL, 1.4187μg/mL 2.1332μg / mL, 2.6234μg/mL, 2.7671μg/mL, both in vitro anti-tumor activity and the presence of cytotoxic significant difference; cells fluorescent double staining results displayed by doxorubicin hydrochloride solution and its solution nanoliposome handle the tumor cells can be observed to a large number was beaded apoptotic and dead cells, while the negative control group, less apoptosis or death. Wherein the doxorubicin hydrochloride nano liposome drug-treated group the number of living cells was significantly less than the same concentration of doxorubicin hydrochloride treatment group; Conclusion: ethanol injection-PH gradient - Motor prepared was stirred out of the doxorubicin hydrochloride nanoliposome the body uniform particle size distribution, high encapsulation efficiency, drug-containing large, the process is simple. Vitro cytotoxicity assays and cell morphology observation, doxorubicin hydrochloride encapsulated in liposomes can more effectively play its cytotoxic promote tumor cell apoptosis.

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CLC: > Medicine, health > Pharmacy > Pharmacology
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