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Lily (Lilium) rich germplasm resources, long history of cultivation, flower rich, beautiful color, the cultivation of a wide range, can be used for cut flowers decoration, garden green, lily bulbs are also edible and medicinal value. Lily is beautiful and elegant and refined, aromatic and pleasant, to be seen as pure light, a symbol of freedom and happiness, the reputation of the king of flower bulbs. China is the hometown of Lily resources, but the lily breeding rather late, lily varieties bred in China with independent intellectual property rarely. And in most parts of northern China cold climate, which makes most of the lily varieties from abroad failed to express its kind. Therefore, breeding in northern China natural climate environmental conditions normal growth of new varieties of lilies, lily breeding of our country has great practical significance. In this study belong to Asiatic, Oriental lily, musk, lily three strains and three wild lily four major categories of 14 lily material test materials for hybrid parents, 14 Lily biological characteristics of the material, and 14 lily pollen viability of the material were measured, combined with the kinship, cold, color, aroma, the green of the length of breeding objectives, on the 14 Lily materials crosses the parent selection, the design includes a total of distant hybridization, species within the hybridization and selfing crosses 112, per crosses pollination to 107, the number of pollinated flowers of 12,000 flowers. Hybridization using conventional direct pollination, cutting stigma pollination trick pollination and reciprocal hybrids the different pollination pollination, pollination combination of 53 after fertilization barriers lead to abortion embryo rescue hybrid seedlings; the same time, 59 hybrid seeds seed dormancy and germination characteristics, medium and peeling treatment, control of conventional sowing Lily Distant unearthed type of hybrid seeds, the germination rate and seedling rate study; Finally, some Asian lily growth and ornamental characteristics of the female parent of the F1 generation lily. The results are as follows: 1. Lancifolium 'and' gross Lily 'cold, strong drought resistance,' Pollyana 'and' essence 'followed by' Sorbonne 'has good potential for breeding, In addition to Longiflorum other 13 varieties of lily pollen germination rate difference was not significant, suitable for use as breeding material. 2.112 crosses, Asiatic lilies affinity for the female parent in Oriental lily; the 'lancifolium' and 'Pollyana' affinity best swollen ovary aspect ratio of about 1.8 to 2.2, seed setting rate the highest. The conventional method of direct cross-pollination for most of the hybrid combination is better; cutting the stigma pollination method can solve a certain extent, distant hybridization before fertilization barriers, but some crosses to be combined with embryo rescue. (4) after the low temperature of the hybrid seeds treated with GA3 can better break dormancy, which cryosphere plot 90d, 400 mg / L GA3 treatment is most effective. 5 seed sterilization method 70% alcohol 60s 2% of NaCl037 to 15 minutes; germination best medium for MS NAA0.1mg / L 6-BA0.5mg / L sucrose 30g / L. Breeding, tissue culture can greatly shorten the life of seed breeding, improve seedling rate. Lily crossbreeding variability is an effective way to breeding.
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