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The China Lily resources important origin of the world, lily breeding in China started relatively late, bred varieties of lily few independent intellectual property rights, to promote the rapid development of China's lily breeding work, this study more than 10 Lily superior varieties of materials to carry out the genetic relationship of parent selection and use of phenotype and SSR technology selected lily varieties, and the results are as follows: (1) 'Justina Zantrijus',' Sheila ',' Elite 'and' Minjiang Lily 'pollen is most suitable for dry refrigerated at 4 ℃, the germination capacity of up to 84 days, respectively, to maintain the optimum storage conditions, 35,28 and 84 days;' Brunello 'pollen is most suitable at 4 ℃, the germination capacity for 70 days ; rather 'Lanzhou lily pollen most suitable for -18 ° C frozen storage, germination capacity for 49 days. Pollen germination rate with storage time prolonged downward trend, but at some stage during storage, there is a marked increase in the phenomenon. (2) at different concentrations and the ratio of the hormone, the impact of the same species, pollen germination rate and pollen germination rate impact of the different varieties of the same concentration and the ratio of the hormone. (3) the 'Lanzhou lily pollen germination rate overall as the treating temperature decreased gradually, the same temperature, the longer the processing time for pollen germination rate is lower. 'White Fox' pollen germination rate of 35 ° C treatment 1hr and 2hrs than 40 ° C, 45 ° C, a temperature of 50 ° C for processing the same time the low germination rate, the processing time of 3hrs, germination rate maximum processing temperature of 50 ° C. 'Sorbonne' pollen can not resist high temperature treatment, the germination rate is suddenly reduced. (4) the Department of Oriental lily hybrid can get citrus hybrids and hybrid embryos, hybridization affinity differences of the different hybrid combinations, some combination did not get the embryos' Willeke Alberti 'as the female parent, the highest affinity,' Topwhite Zanler as female-white 'affinity for the worst; Lily Department of hybridization were not hybrid embryos. (5) different hybrid embryos of different hormone type and concentration requirements, 'Sheila' × 'Francia' hybrid embryos, the best medium was MS 0.1mg.L-1 NAA 0.5mg.L-16-BA 40g. L-1 sucrose 6g.L-1 agar, 'Ceb Dazzle' × 'Elite' hybrid embryos, the best medium: MS 0.01mg.L-1 NAA 1.0mg.L-1BA 40g.L-'sucrose 6g . L-1 agar. (6) phenotype and SSR-based genetic relationship study showed that between the phenotypic traits Euclidean distance matrix and SSR markers genetic distance matrix is ??not significantly negatively correlated with a correlation coefficient of -0.0022 (P = 0.9737), prove select phenotypic traits can not accurately reflect the genetic variation of the relatives of similar material. (7) From the results of cross-pollination, hybridization affinity with SSR markers genetic distance is not necessarily linked. When the SSR markers the genetic distance is greater than 1.0, each combination can not be obtained the seed embryos.
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