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Lily (Lilium spp.) Is a general term for all kinds of the lily family (Liliaceae), a subclass of monocots Lilium (Lilium), is one of the world-famous cut flowers, lily breeding work long hours, difficult hybridization. Oriental lily of the Siberian (2n = 2x = 24), OT hybrid system lily Manissa (3n = 36), Robinna (3n = 36) for the material, from the parental pollen vitality determination, conventional hybridization test, hybrid offspring embryos to save and culture, hybrids cytological identification of the four aspects of research, the results are as follows: 1, Pollen germination Determination pollen viability parents. The results are as follows: the vitality of the Oriental lily Siberian pollen highest average germination rate of 87%, while of OT Department the lily Manissa and the Robinna complex genetic background, pollen development degree of inconsistency low pollen viability of 3% and 18%, respectively. . Using the germination method to determine the initial pollen viability. Practical application requires a combination of pollen determination methods of pollen viability comprehensive test to guide parent selection, is the key to hybrid success rate. 2, in the hybridization experiment, using conventional method for different combinations of the field pollination experiment. The results show that: the combination (Siberia × Manissa) get with (Manissa X Robinna), a small amount of embryo offspring. Combination as the female parent to Huangtian Ba, Robina just a little swollen capsule, but did not get the embryo fruit. Speculated that there are two possibilities: First, the OT Department lily stylar longer makes pollen tubes can not be effectively extends the style, the conventional method is not suitable for the cross-pollination of the female parent OT Department, may consider cutting style method to The overcome pollination of obstacles before; selected male parent and female parent affinity bad. Screened hybrids suitable embryo rescue culture conditions, the results showed that: about 60 days mature embryos stripping embryo culture better training methods; suitable medium for: MS GA3O.2 NAAO.1 2% Sugar 0.05% PVP , GA3 conducive to shorten the the embryo germination time, NAA is conducive to the growth of an embryo. The embryo germination early to give proper illumination is conducive to the germination of the embryo, average germination time in about two days in advance. 4, respectively, select the F1 generation of lines made chromosomal speciation analysis, the results showed that: two combinations hybrids has been reset and exchange of chromosomes, parental chromosome morphology, the preliminary judgment F1 hybrid is the offspring of parental income test.
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