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Analysis of Paulownia Plants with Witches’ Broom Tissue Culture Seedling Treated with Rifampicin Using SSR Technique

Author: ChangLi
Tutor: YuFangYuan；FanGuoQiang
School: Nanjing Forestry University
Course: Forest cultivation
Keywords: Paulownia Witches' broom Phytoplasma SSR Rifampicin
CLC: S763.7
Type: Master's thesis
Year: 2009
Downloads: 34
Quote: 1
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Abstract


Firstly, the transfer amplification method to filter out the SSR primer suitable for paulownia, Paulownia SSR reaction system, rifampicin of witches 'broom Paulownia witches' broom Paulownia fortunei and arbuscular disease in this study on the basis of the the morphology of of Yuza One Paulownia phytoplasma content and three paulownia healthy tissue culture, suffering from the arbuscular disease tissue culture and rifampicin treatment with different concentrations of suffering witches' broom tissue culture SSR analysis, the results are as follows:. screened from other woody plants, 416 SSR primer pairs 73 pairs in Paulownia amplification products amplified product bar with clear, reproducible primers in Paulownia share on the successful amplification primers primer ratio was 20.67%. The results show that only the primer PMGC684 exhibit polymorphism, the paulownia 9503 to 11 clones separate varieties District, 72 pairs of primers not exhibit polymorphism analysis of SSR 12 Paulownia clones. 2 using 73 pairs of SSR primers screened were suffering from the the arbuscular disease Paulownia tomentosa and health Paulownia tomentosa, suffering from 1 Yuza Paulownia witches' broom and health Yuza One paulownia, suffering from of arbuscular disease Paulownia fortunei and health Paulownia fortunei the genomic DNA for PCR amplification. The results showed that 73 SSR primer pairs paulownia three health and suffering from the of arbuscular disease Paulownia amplification results consistent. Treated with different concentrations of rifampicin three suffering from the tissue culture of paulownia witches' broom, and the results show that the three paulownia tissue culture of the arbuscular symptoms gradually disappear with increasing rifampicin concentrations in a healthy state transformation, but the rooting time is gradually postponed, rifampicin concentration of 150mg · L -1 , continue to increase the concentration of seedlings growing gradually worse, indicating rifampicin role in phytoplasma the also host Paulownia generated certain toxic effects. By nested PCR of different concentrations of rifampicin treatment suffering witches 'broom Paulownia tomentosa suffering of arbuscular disease Paulownia fortunei and suffering the phytoplasmas content of witches' broom, 1 Yuza Paulownia Seedlings Tissue detect, the results show that Paulownia body, rifampicin concentrations increase phytoplasma content decreased when rifampicin concentration of 150mg L -1 to detect the presence of less than phytoplasma. Using 73 pairs of SSR primers were screened out of the suffering of different concentrations of rifampicin treatment witches' broom Paulownia tomentosa, suffering from the the arbuscular disease Yuza One paulownia and suffering from the of arbuscular disease Paulownia fortunei Paulownia genomic DNA for PCR amplification The results show that each processing the amplification results in a 73-bit point.

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CLC: > Agricultural Sciences > Forestry > Forest Protection > Forest Pest and Disease Control > All kinds of tree pests and diseases and their prevention
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