|
Objective: To detect TRF1 and TRF2 protein expression in cervical cancer and intraepithelial neoplasia ; analysis of high-risk types of human papillomavirus (HPV) infection with TRF1 , TRF2 protein expression . METHODS: In situ hybridization and immunohistochemical methods to detect high-risk HPV and TRF1 , TRF2 protein expression in 15 cases of normal cervical epithelium , 36 cases of cervical intraepithelial neoplasia (CIN) and 35 cases of cervical squamous cell carcinoma . The result : high-risk HPV in normal cervical epithelium , CIN , cervical squamous cell carcinoma positive expression rate was 20.0% ( 3 / 15 ) , 63.9% ( 23 / ??36 ) , 97.1% ( 34 / 35 ) . HPV infection rate of CIN and cervical squamous cell carcinoma group compared with the normal group , there were significant differences ( P lt; 0.01 ) . TRF1 in normal cervical epithelium , CIN, cervical squamous cell carcinoma , the positive expression rates were 86.7% ( 13 / 15 ) , 63.9% ( 23 / ??36 ) , 40.0% ( 14 / 35 ) . Which cervical squamous cell carcinoma and CIN group , compared to the normal group , CIN Ⅰ group and CIN Ⅲ group showed a significant difference ( P lt; 0.01 ) . TRF2 in normal cervical epithelium , CIN, cervical squamous cell carcinoma , the positive expression rate was 33.3 % (5 / 15 ) , 52.8% ( 19 / 36 ) , 80.0% ( 28 / 35 ) . Which cervical squamous cell carcinoma and CIN group , compared to the normal group , were significantly different ( P lt; 0.01 ) . The intensity of high-risk HPV infection with TRF1 protein expression negatively correlated (Rs = -0. 302, P lt; 0.05). HPV infection and TRF2 protein expression intensity positively correlated ( Rs = 0. 452 , P lt ; 0 , 01) . Conclusion : TRF1, TRF2 plays an important role in the development of cervical cancer . Downregulation of TRF1 and TRF2 upregulation with high-risk HPV infection in cervical cancer are closely related.
|