Dissertation > Excellent graduate degree dissertation topics show

The Empirical Study of Exogenous Vascular Endothelial Growth Factor Combining Hyperbaric Oxygen Treatment on the Survival of Free Skin Flaps in Rats

Author: WangJingDong
Tutor: WangJiangNing
School: Dalian Medical University
Course: Surgery
Keywords: Vascular endothelial growth factor Hyperbaric oxygen Random flap Vascular density
CLC: R622
Type: Master's thesis
Year: 2009
Downloads: 40
Quote: 0
Read: Download Dissertation

Abstract

Objective: Repair Medical research hotspot in recent years, more and more attention with the progress of society and the development of medicine, and its importance, in general, the repair process is a common clinical variety of wounds and disease during the treatment outcome. The ideal repair outcome should by scar tissue anatomical structures and physiological functions of complete rehabilitation. However, in the actual repair process, many organizations and external factors from the cellular, molecular, and genetic level affect or interfere with the repair process, so that the repair process is difficult to repair out of control in accordance with the wishes of the people. In the field of Plastic and Reconstructive Surgery, the random pattern flap is one of the most frequently used method of repair. Flap is not well-known vascular blood supply subcutaneous capillary network alone, flap design is often required to follow a principle that flap length, width ratio limit for the length: width = 2 : a rich blood supply in some local areas, up to 3:1. Outside this range, the distal flap easily occur avascular necrosis. Random flap transplantation has a failure rate of 10% -20%, when using this extra-long flap must often required to do a \How can this extra-long flap once the transfer is successful, is a subject worthy of study. This study aims to explore the super random flap in the local injection of vascular endothelial growth factor (VEGF) whether the combined application of hyperbaric oxygen (Hyperbaric Oxygen, HBO) can improve flap microcirculation, increase the blood supply of the flap to improve flap survival rate. MATERIALS AND METHODS: 1 Packet: 12 adult rats divided into two groups in accordance with the principle of random allocation, A (experimental group): 6; B group (the control group): 6. Flap preparation: the rat abdominal preparation of random flap, its aspect ratio as 3:1 (3X1cm). After intravenous anesthesia with 5% sodium pentobarbital (10mg/kg), hair removal, skin preparation, after disinfection, remote from the prefabricated flap separation in the the meat film with subdermal vascular network layer between skin random pattern flap flap prepared 3, after four days, 10 days, 12 days, respectively, forming the flap far in the proximal segment do PU value determination. 12 days the rats were killed, cut a flap specimen and immediately with 4% PFA (poly formaldehyde) were fixed, making wax block, serial sections to do the conventional staining organization morphological changes, immunohistochemistry method (SP) proliferating cell nuclear antigen (PCNA) and microvessel density (MVD) detection. The establishment of a database and the results were analyzed by ANOVA with SPSS13.0. Results: ① PU value measurement of the flap formation of two sets of values ??were no significant differences. The same period in the two groups in different periods after the formation of the flap flap showed distal perfusion worse than the middle and proximal blood supply of the proximal foot two groups flap formation in different parts of the value of the difference in the proximal segment sex was not statistically significant, there are significant differences in the distal value. ② HE staining: A group survived the subcutaneous tissue of the flap has a certain degree of edema, epidermal hyperplasia, under the skin, a large area of ??granulation tissue, cell proliferation lot of new capillary formation. Group B survived flap subcutaneous tissue edema, scattered subcutaneous hemorrhage, neovascularization and granulation tissue distribution range is significantly less than the A group. The distal flap fully necrosis, loss of cell morphology. The ③ PCNA detection: A group survived the flap in the active cell proliferation (PI = 63%), a lot of pale-brown staining of proliferating cell nuclear basal cells hyperplasia is particularly evident in the proliferation of granulation tissue with a lot of new capillaries, group B expression than The weak (PI = 20%), the positive cells were scattered neovascularization. There are significant differences in the two sets of flaps. ④ flap microvascular density (MVD) detection: A group be brownish yellow staining of vascular endothelial cells, end vascular distribution intensive MVD = 45 / cm2, group B blood vessels less distributed, sparse pale-brown staining of vascular endothelial cells MVD = 20 / cm2. The two groups have significant differences (P lt; 0.001) MVD. Conclusion: 1, postoperative local injection of vascular endothelial growth factor (VEGF) combined application of hyperbaric oxygen (Hyperbaric Oxygen, HBO) therapy can significantly reduce the rat abdominal flap necrosis rate arbitrarily, promote flap .2, HBO can improve flap density of blood vessels in the tissue, the local injection of vascular endothelial growth factor (VEGF) combined application of hyperbaric oxygen to promote the formation of random flap in tiny blood vessels.

Related Dissertations

CLC: > Medicine, health > Surgery > Plastic Surgery (repair surgery ) > Plastic surgery school
© 2012 www.DissertationTopic.Net Mobile