Dissertation > Excellent graduate degree dissertation topics show

Expression and Clinical Significance of CD44v6、MMP-9 and Oct4 in Breast Cancet

Author: LinMin
Tutor: LiLianHong
School: Dalian Medical University
Course: Pathology and Pathophysiology
Keywords: breast carcinoma CD44v6 MMP-9 Oct4 invasion
CLC: R737.9
Type: Master's thesis
Year: 2009
Downloads: 122
Quote: 0
Read: Download Dissertation

Abstract


Objective:The expression of CD44v6, MMP-9 and Oct4 in breast cancer and the pathologic characteristics of breast cancer were detected to investigate the effectiveness of CD44v6, MMP-9 and Oct4 in the development, invasion and metastasis of breast cancer. The relation of these three factors was also discussed. The aim was to understand the mechanism involving breast cancer and breast stem cells, and to provide theoretical evidences for clinical breast cancer diagnosis and treatment.Methods:The expression of CD44v6, MMP-9 and Oct4 proteins were detected in 37 cases of breast invasive ductal carcinoma by S-P immunohistochemical technique. The results were assessed by the method in accordance with Liangzhong Xu’s. The patients’age, tumor size and lymphatic matastasis were analyzed by clinical pathologic method. Statistical analysis was performed by SPSS 11.5 statistical software. Data was analyzed by Fisher probabilities in 2×2 table, the chi-square test, and Spearma interclass correlation analysis.Results:1. The expression of CD44v6, MMP-9 and Oct4 were respectively located in cell membrane, cytoplasm and cytoplasm in breast invasive ductal carcinoma, positive expression were respectively 54.1%, 59.5% and 43.2%.2. The positive expression of CD44v6 and MMP-9CD44v6 were correlated with tumor grading and lymph node metastasis (P<0.05), and were not evidently correlated with patients’age, tumor size, positive or negative ER and PR (P>0.05).3. Oct4 was correlated with tumor grading( P < 0.05) , and not evidently correlated with patients’age, tumor size, positive or negative ER and PR (P>0.05).4. By Spearman interclass correlation analysis, the expression of CD44v6 was directly correlated with that of MMP-9(rs=0.703,P<0.01),the expression of Oct4 was not evidently correlated with those of CD44v6 and MMP-9 (P >0.05).Conclusion:1.The expression of CD44v6 was different due to whether there was axillary node metastasis in breast cancer or not, and tumor grading difference, that is, CD44v might promote axillary node metastasis of breast cancer and the expression enhanced with tumor grading increasing.2. The expression of MMP-9 enhanced obviously with tumor grading increasing, which showed that poorly differentiated cancer cells were of high potential to induce the expression of MMP-9, and therefore was of high invasion ability and took an important role in tumor growth, invasion and metastasis.3. The expression of CD44v6 in tumor cells indicated that there were a number of multipotential poorly differentiated or undifferentiated tumor cells with stem cell property in breast carcinoma tissues with different pathologic grading. The positive expression intensity of CD44v6 in breast carcinoma tissues increased with carcinoma cell differentiation level descending. The lower tumor cell differentiation level, the more tumor cell genetic structure and phenotype were similar to primitive immature stem cells, which illustrated that breast carcinoma tissues with relative high malignant potential had more tumor cells with stem cell property.4.The expression of CD44v6 being significantly directly correlated with that of MMP-9, which indicated that both of CD44v6 and MMP-9 played an important part in the progress of the metastasis of breast invasive ductal carcinoma, and they could serve as important molecular makers of invasion and metastasis of breast carcinoma. Blocking the expression of CD44v6 and MMP-9 could provide a direction to breast cancer therapy. The mechanism might be that because of CD44v6 and MMP-9, tumor cells had the ability to detach each other easily and degrade basement membrane and extracellular matrix to make tumor cells break through various barriers in invasion and metastasis progress, for example, basement membrane of epithelial cells, basement membrane of blood vessels, and to make cell adhesion ability enhanced and metastasis easily occur.

Related Dissertations

  1. Clinical Significance of STAT3 and MMP-2 in Hepatocellular Carcinoma.,R735.7
  2. Effect of Invasive Plants (Conyza Sumatrenss and Alternanthera Philoxeroides) on Soil Carbon and Nitrogen Processes,X173
  3. Expression of MMP-9 and p63 in Oral Squamous Cell Carcinoma and Clinical Significance,R739.8
  4. Experimental Study on 420nm Intense Pulse Light in Animal Acne Model,R758.733
  5. Initial Research on the Expression and the Significance of Cx-43、 Caspase-3 and MMP-2 in Endothelial Cells in Atherosclerosis in Human Coronary Artery,R541.4
  6. Old Ⅲ Colorectal Cancer Survival after Radical Analysis and Evaluation of Chemotherapy,R735.3
  7. Study on Expression of MMP-8 on Experimental Periapial Periodontitis is Infected by Enterococcus Faecalis,R781.34
  8. The Influence of Cold Self-bloodcardioplegia to MMP-2 Concentration for Infant with Cardiopulmulnary Bypass,R726.5
  9. The Experimental Study of Atorvastatin with Neuroprotective Effects by Inhibiting MMP-9 and Upregulating TIMP-1 Expression in Rat Stroke Model of Focal Cerebral Ischemia/Reperfusion,R743.33
  10. Galectin-3, MMP-2 and MMP-9 expression in colorectal carcinoma and their correlation,R735.3
  11. MMP-2, VEGF, CD105, Ki67 in small cell lung carcinoma and prognosis of,R734.2
  12. Relationship between Epithelial-mesenchymal Transition Induced by Hypoxia and Invasive Ability in Hepatocellular Carcinoma Cells,R735.7
  13. The Role and Mechanism of SDF-1/CXCR4 Axis in Small Cell Lung Cancer Invasion and Metastasis,R734.2
  14. Role and Mechanism of CD40 Signal on Lung Cancer Cell Line H1299 Proliferation、Migration and Invasion,R734.2
  15. Expression and Clinical Significance of LOX Mrna, Mmp-2mrna and CD24mRNA in Prostate Cancer,R737.25
  16. Effects of X-ray Radiation and Combined with CCR7-siRNA on Proliferation, Invasion and Migration in Lung Carcinoma A549 Cells in Vitro,R734.2
  17. The Effects and Underlying Mechanisms of S-allyl L-cysteine on the Retina Injury Subjected to Ischemia/reperfusion,R962
  18. Clinical Value and Expression of COX-2, MMP-9 and VEGF in Colorectal Cancer,R735.3
  19. A Study of the Expression of MMP-1 and TIMP-1 at the Enthesiopathy and the Intervention Effects by Acupuncture,R87
  20. The Expression of plexin-B2 in Tumor and the Molecular Mechanism of plexin-B2 Promoting Carcinoma Invasion and Metastasis,R737.33
  21. The Effect of Gambogic Acid on the Growth Activity and Invasion in Renal Cell Carcinoma 786-O Cells,R737.11

CLC: > Medicine, health > Oncology > Genitourinary tumors > Breast tumor
© 2012 www.DissertationTopic.Net  Mobile